Gentamicin (50 mg/mL)

Citações e referências (13)

Gibco™

Gentamicin (50 mg/mL)

Name: Gentamicin (50 mg/mL)
SKU: 15750060
Price: 563.51 BRL

Gentamicin sulfate is a water-soluble antibiotic originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30SLeia mais

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Número do catálogo	Quantity
15750060	10 mL
15750078	10 x 10 mL

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Número do catálogo 15750060

Preço (BRL)

563,51

Each

Adicionar ao carrinho

Quantity:

10 mL

Preço (BRL)

563,51

Each

Adicionar ao carrinho

Gentamicin sulfate is a water-soluble antibiotic originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Gibco™ Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used to prevent the contamination of cell cultures by bacteria. The recommended working concentration ranges from 0.5 to 50 μg /mL.

We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.

Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures.

Dual-Site cGMP Manufacturing
Gibco™ Gentamicin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco™ Gentamicin product made in our Scotland facility (15750-037). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

For Research Use Only. Not for use in diagnostic procedures.

Especificações

Concentration50 mg/mL

Recommended StorageStorage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

Shipping ConditionRoom Temperature

Sterile FilteredYes

Validated ApplicationPrevention of Cell Culture Contamination

Physical FormLiquid

Quantity10 mL

TypeGentamicin

Unit SizeEach

Frequently asked questions (FAQs)

Once Gentamicin (50 mg/mL; Cat. No. 15750060) is added to the cell culture medium, can this resulting medium, including gentamicin, be frozen and thawed before using it in cell culture without affecting the performance of the antibiotic?

Yes, gentamicin can be frozen in a prepared medium. However, we don't have any stability data on this process with media not made here in our facility. So, you would have to determine the effectiveness of the product upon thawing empirically.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citações e referências (13)

Citações e referências

Abstract

A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.

Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T

Journal:Experimental cell research

PubMed ID:38499143

Biosynthetic studies of A2E, a major fluorophore of retinal pigment epithelial lipofuscin.

Authors: Ben-Shabat Shimon; Parish Craig A; Vollmer Heidi R; Itagaki Yasuhiro; Fishkin Nathan; Nakanishi Koji; Sparrow Janet R;

Journal:J Biol Chem

PubMed ID:11756445

'We have examined questions related to the biosynthesis of A2E, a fluorophore that accumulates in retinal pigment epithelial cells with aging and in some retinal disorders. The use of in vitro preparations revealed that detectable levels of A2-PE, the A2E precursor, are formed within photoreceptor outer segments following light-induced release ... More

Growth-dependent Regulation of Mammalian Pyrimidine Biosynthesis by the Protein Kinase A and MAPK Signaling Cascades.

Authors: Sigoillot Frederic D; Evans David R; Guy Hedeel I;

Journal:J Biol Chem

PubMed ID:11872754

'The carbamoyl phosphate synthetase domain of the multifunctional protein CAD catalyzes the initial, rate-limiting step in mammalian de novo pyrimidine biosynthesis. In addition to allosteric regulation by the inhibitor UTP and the activator PRPP, the carbamoyl phosphate synthetase activity is controlled by mitogen-activated protein kinase (MAPK)- and protein kinase A ... More

The potent anti-HIV protein cyanovirin-N contains two novel carbohydrate binding sites that selectively bind to Man(8) D1D3 and Man(9) with nanomolar affinity: implications for binding to the HIV envelope protein gp120.

Authors: Bewley C A; Otero-Quintero S

Journal:J Am Chem Soc

PubMed ID:11457139

'Cyanovirin-N (CVN) is a monomeric 11 kDa cyanobacterial protein that potently inactivates diverse strains of human immunodeficiency virus (HIV) at the level of cell fusion by virtue of high affinity interactions with the surface envelope glycoprotein gp120. Several lines of evidence have suggested that CVN-gp120 interactions are in part mediated ... More

Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.

Authors:Avci FY, Li X, Tsuji M, Kasper DL,

Journal:Nat Protoc

PubMed ID:23196974

Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized ... More

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