Gentamicin (50 mg/mL)

Citações e referências (5)

Gibco™

Gentamicin (50 mg/mL)

Name: Gentamicin (50 mg/mL)
SKU: 15750078
Price: 4.587.59 BRL

Gentamicin sulfate is a water-soluble antibiotic originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30SLeia mais

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Número do catálogo	Quantity
15750078	10 x 10 mL
15750060	10 mL

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Número do catálogo 15750078

Preço (BRL)

4.587,59

Each

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Quantity:

10 x 10 mL

Preço (BRL)

4.587,59

Each

Adicionar ao carrinho

Gentamicin sulfate is a water-soluble antibiotic originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Gibco™ Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used to prevent the contamination of cell cultures by bacteria. The recommended working concentration ranges from 0.5 to 50 μg /mL.

We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.

Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures.

Dual-Site cGMP Manufacturing
Gibco™ Gentamicin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco™ Gentamicin product made in our Scotland facility (15750-037). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

For Research Use Only. Not for use in diagnostic procedures.

Especificações

Concentration50 mg/mL

Recommended StorageStorage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

Shipping ConditionRoom Temperature

Sterile FilteredYes

Validated ApplicationPrevention of Cell Culture Contamination

Physical FormLiquid

Quantity10 x 10 mL

TypeGentamicin

Unit SizeEach

Frequently asked questions (FAQs)

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citações e referências (5)

Citações e referências

Abstract

Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.

Authors:Wiedemann A, Rosselin M, Mijouin L, Bottreau E, Velge P,

Journal:J Biol Chem

PubMed ID:22810232

'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion ... More

Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.

Authors:Avci FY, Li X, Tsuji M, Kasper DL,

Journal:Nat Protoc

PubMed ID:23196974

Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized ... More

A three-dimensional co-culture system to investigate macrophage-dependent tumor cell invasion.

Authors:Dwyer AR, Ellies LG, Holme AL, Pixley FJ

Journal:J Biol Methods

PubMed ID:31453214

'Macrophages infiltrate cancers and promote progression to invasion and metastasis. To directly examine tumor-associated macrophages (TAMs) and tumor cells interacting and co-migrating in a three-dimensional (3D) environment, we have developed a co-culture model that uses a PyVmT mouse mammary tumor-derived cell line and mouse bone marrow-derived macrophages (BMM). The Py8119 ... More

Characterizing the Antimicrobial Function of a Dairy-Originated Probiotic,

Authors:Nair DVT, Kollanoor Johny A

Journal:Front Microbiol

PubMed ID:30050507

'Antimicrobial potential of a dairy-origin probiotic bacteria,'

Palbociclib treatment alters nucleotide biosynthesis and glutamine dependency in A549 cells.

Authors:Conroy LR, Lorkiewicz P, He L, Yin X, Zhang X, Rai SN, Clem BF

Journal:Cancer Cell Int

PubMed ID:32624705

Aberrant activity of cell cycle proteins is one of the key somatic events in non-small cell lung cancer (NSCLC) pathogenesis. In most NSCLC cases, the retinoblastoma protein tumor suppressor (RB) becomes inactivated via constitutive phosphorylation by cyclin dependent kinase (CDK) 4/6, leading to uncontrolled cell proliferation. Palbociclib, a small molecule ... More