dNTP Mix (10 mM ea)
dNTP Mix (10 mM ea)
Citações e referências (1)
Invitrogen™

dNTP Mix (10 mM ea)

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is atLeia mais
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Número do catálogoQuantity
18427013100 μL
184270895 x 1 mL
184270881 mL
Número do catálogo 18427013
Preço (BRL)
948,01
Each
Adicionar ao carrinho
Quantity:
100 μL
Request bulk or custom format
Preço (BRL)
948,01
Each
Adicionar ao carrinho

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes including: polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Features include:
• Chemically synthesized
• >99% purity confirmed by HPLC
• Stable for 2 years at –20°C
• Free from qPCR, PCR, reverse transcription inhibitors
• Free of DNases and RNases
• Free of human and E. coli DNA

Applications
• qPCR, RT-qPCR
• PCR, RT-PCR, cDNA synthesis
• High-fidelity and long-range PCR
• Isothermal amplification
• DNA labeling
• Cloning
• Sanger and next-generation sequencing (NGS)

Thermo Fisher Scientific offers a wide variety of dNTPs in different concentrations, volumes, and formats.

For Research Use Only. Not for use in diagnostic procedures.
Especificações
Quantity100 μL
Shipping ConditionApproved for shipment on Wet or Dry Ice
Concentration10 mM
For Use With (Application)Standard PCR, RT-PCR, Real Time PCR (qPCR)
FormLiquid
Label or DyeUnlabeled
Unit SizeEach
Conteúdo e armazenamento
dNTP Mix, contains 10 mM each of dATP, dCTP, dGTP, and dTTP (100 μL)

Store at –20°C.

Frequently asked questions (FAQs)

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

Why do some dNTP mixes contain dTTP and others TTP?

Thymine is normally found as a deoxyribose. Thus, TTP and dTTP are essentially the same thing. Both types of mixes can be used in PCR reactions

Why does the annealing temperature have to be above 55 degrees C, when using the GeneAmp PCR Carryover Prevention kit (Cat. No. N808-0068) or any kit containing AmpErase UNG (Cat. No. N808-0096)?

Uracil-N-glycosylase (UNG) has activity in the temperature range of 4 degrees C to 55 degrees C, and is not completely inactivated by incubation at 95 degrees C. It is recommended that the annealing temperature be at or above 55 degrees C when using this enzyme with any of our PCR or Sequence Detection Systems kits to avoid possible degradation of newly synthesized dU-containing PCR products by residual UNG activity. For this reason, it is also recommended that the post-PCR soak be maintained at 72 degrees C (instead of 4 degrees C) if analysis of the PCR product will be performed using a gel-based method.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

What concentration of dNTPs is typically used in a PCR amplification?

The four dNTPs (dATP, dCTP, dTTP, dGTP) are used at a final concentration of 200 µM each or a total of 0.8 mM in standard amplifications. For PCR amplifications that require high fidelity, lower dNTP concentrations (on the order of 10-50 µM) work optimally.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Describe the amplified product produced by the control reagents in the GeneAmp Gold PCR Reagent Kit with Multiplex Control (Cat. No. 4312778).

The control primers generate a 300 and a 500 bp amplicon from the lambda control DNA. The Lambda Multiplex Control primers, as well as the Lambda Control DNA are provided in this kit.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Citações e referências (1)

Citações e referências
Abstract
Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays.
Authors: Bernstein Jonathan A; Khodursky Arkady B; Lin Pei-Hsun; Lin-Chao Sue; Cohen Stanley N;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12119387
Much of the information available about factors that affect mRNA decay in Escherichia coli, and by inference in other bacteria, has been gleaned from study of less than 25 of the approximately 4,300 predicted E. coli messages. To investigate these factors more broadly, we examined the half-lives and steady-state abundance ... More