Biotin-14-dATP

Biotin-14-dATP is a patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker.Leia mais

Número do catálogo	Quantity
19524016	50 nmol

Número do catálogo 19524016

Preço (BRL)

3.844,71

Adicionar ao carrinho

Quantity:

50 nmol

Preço (BRL)

3.844,71

Adicionar ao carrinho

Biotin-14-dATP is a patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP (1). Biotin-14-dATP can be added to the 3´ end of DNA using terminal deoxynucleotidyl transferase (2). The amount supplied is sufficient for labeling up to 50 μg of DNA by nick translation.

Performance and Quality Testing: Purity is evaluated by reverse-phase HPLC analysis.

For Research Use Only. Not for use in diagnostic procedures.

Especificações

FormatLiquid

Labeling MethodDirect Labeling

Product TypeLabeled Nucleotide

Quantity50 nmol

Shipping ConditionApproved for shipment on Wet or Dry Ice

Concentration0.4 mM

Label or DyeBiotin and Other Hapten

Unit SizeEach

Conteúdo e armazenamento

Biotin-14-dATP is supplied at 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C.

Citações e referências (1)

Citações e referências

Abstract

Isolation and characterization of the human cytochrome P450 CYP1B1 gene.

Authors: Tang Y M; Wo Y Y; Stewart J; Hawkins A L; Griffin C A; Sutter T R; Greenlee W F;

Journal:J Biol Chem

PubMed ID:8910454

'Previously, we identified a novel human cytochrome P450 cDNA that is inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and represents the first member of a new subfamily designated cytochrome P4501B1 (CYP1B1; Sutter, T. R., Tang, Y. M., Hayes, C. L., Wo, Y. P., Jabs, E. W., Li, X., Yin, H., Cody, C. W., ... More