Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible

Citações e referências (1)

Thermo Scientific™

Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible

This BCA Protein Assay Kit is a reducing agent-compatible version of our popular Pierce BCA Protein Assay. The kit enablesLeia mais

Número do catálogo	Quantity
23250	275 mL

Número do catálogo 23250

Preço (BRL)

2.551,77

Adicionar ao carrinho

Quantity:

275 mL

Request bulk or custom format

Preço (BRL)

2.551,77

Adicionar ao carrinho

This BCA Protein Assay Kit is a reducing agent-compatible version of our popular Pierce BCA Protein Assay. The kit enables you to measure protein concentration in samples that contain the disulfide reducing agents.

Compare all available BCA protein assays ›

Features of the BCA Protein Assay Kit—Reducing Agent Compatible include:
• Compatibility—assay samples that contain up to 5 mM DTT, 35 mM BME, or 10 mM TCEP
• BCA technology—only a slight modification of the standard BCA Protein Assay protocol (15-min incubation with Compatibility Reagent); no precipitation steps required
• Sample volume—requires only 25 μL (standard kit) or less than 10 μL (microplate kit) of sample
• Colorimetric—measure with a standard spectrophotometer or plate reader at 562 nm
• Excellent uniformity—exhibits less protein-to-protein variation than dye-binding methods
• Assay range—linear working range for BSA of 125 to 2000 μg/mL

The BCA Protein Assay Kit—Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, BME, or TCEP. This reducing agent-compatible BCA kit extends the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

Related products
Pierce Microplate BCA Protein Assay Kit - Reducing Agent Compatible
96-Well Plates for Pierce BCA-RAC Assay

For Research Use Only. Not for use in diagnostic procedures.

Especificações

AssayBCA Assay

DescriptionPierce BCA Tube Protein Assay Kit, Reducing Agent Compatible (RAC)

For Use With (Application)Solution-based Detection, Absorbance

For Use With (Equipment)Spectrophotometer, Microplate Reader

Product LinePierce

Product TypeProtein Quantitation Assay

Quantity275 mL

SpecificityNot Target-Specific

Sufficient For250 Tube Assays

Detection MethodColorimetric

Unit SizeEach

Conteúdo e armazenamento

Sufficient For: 250 tube assays with 25 μL samples
• BCA Reagent A, 250 mL
• BCA Reagent B, 25 mL
• Compatibility Reagent, 10 x 20 mg
• Reconstitution Buffer, 15 mL
• Albumin Standard Ampules, 2mg/mL, 10 x 1 mL

Store at room temperature

Frequently asked questions (FAQs)

Which Thermo Scientific protein assay is the best or the most reliable?

The choice of protein assay is dependent on preferences related to assay speed, accuracy and sensitivity, as well as interfering substances in the sample to be assayed. BCA has less protein-to-protein variation, is compatible with most detergents, and has larger working range. Pierce Bradford Plus Protein Assay Kit (Cat. Nos. 23236, A55866) is compatible with reducing sugars, is more sensitive and is faster and easier to use. For a comparison of different protein assays and compatible reagents, see our Tech Tip: Protein Quantitation Assay Compatibility Table (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0068-Protein-assay-compatibility.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Can the compatibility reagent in the BCA-RACE kit be stored after reconstitution and reused?

The reconstituted compatibility reagent is stable for 8 hours when stored at 4 degrees C protected from light.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many assays can be performed with each BCA-RAC kit?

The BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23250) has sufficient reagents for 250 samples or standards of 25µL each, and the Microplate BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23252) can be used to assay 1,000 samples or standards of 9µL each.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I increase the volumes of the sample and compatibility reagent in the microplate assay when using the BCA-RAC?

No. Increasing the sample and compatibility reagent volumes will require increasing the BCA working reagent, resulting in a volume that exceeds the well capacity. There must be space remaining in the well to allow mixing and prevent the solution from contacting the sealing tape.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What formats are available for the BCA-RAC?

There are two different formats: the BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23250) for microcentrifuge tube (1.5ml) assays and Microplate BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23252) for 96-well plates. The tube assay is compatible with 5mM DTT, 10mM TCEP and 35mM 2ME, and the microplate assay is compatible with 5mM DTT, 10mM TCEP and 25mM 2ME.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible FAQs

Citações e referências (1)

Citações e referências

Abstract

Measurement of protein using bicinchoninic acid.

Authors:Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC

Journal:Anal Biochem

PubMed ID:3843705

Bicinchoninic acid, sodium salt, is a stable, water-soluble compound capable of forming an intense purple complex with cuprous ion (Cu1+) in an alkaline environment. This reagent forms the basis of an analytical method capable of monitoring cuprous ion produced in the reaction of protein with alkaline Cu2+ (biuret reaction). The ... More