Pierce™ Glycoprotein Staining Kit

The Pierce Glycoprotein Staining Kit is a convenient, fast, and sensitive colorimetric kit for staining glycosylated proteins in polyacrylamide gels or nitrocellulose membranes using the periodic acid-Schiff (PAS) method.

Features:
• Specific for glycoproteins—PAS method specifically detects glycosylated proteins with sialic acid and other oxidizable carbohydrate groups
• Protocol—the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours
• Colorimetric—glycoproteins are detected as magenta bands against a faint pink or colorless gel background
• Qualitative staining—detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625 ng avidin and 0.16 μg horseradish peroxidase per band
• Controls included—complete kit includes positive and negative control proteins (HRP and STI, respectively).

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The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions.