Trypsin-EDTA (0.05%), phenol red

Citações e referências (2)

Gibco™

Trypsin-EDTA (0.05%), phenol red

Name: Trypsin-EDTA (0.05%), phenol red
SKU: 25300096

Gibco™ Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestiveLeia mais

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Número do catálogo	Quantity
25300096 conhecido também como 25300-096	20 x 100 mL
25300104	10 x 500 mL

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Número do catálogo 25300096

conhecido também como 25300-096

Preço (BRL)

Quantity:

20 x 100 mL

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Gibco™ Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco™ trypsin solutions and recommended dissociation conditions.

We offer a variety of trypsin formulations and animal origin-free TrypLE™ reagent that feature:

• Quality Testing
• Documented Traceability
• Dual-site cGMP Manufacturing

This trypsin solution is modified as follows:

With
• EDTA
• Phenol Red

The complete formulation is available.

Quality Testing
Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and precursor lots are tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented Traceability
We can provide detailed documentation to meet your regulatory needs. Gibco™ Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP Manufacturing and Quality System
Gibco™ Trypsin is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ Trypsin solution made in our Grand Island, New York facility (25300-120). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Especificações

Cell TypeMammalian Cells

ChelatorsEDTA

Concentration1 X

Quantity20 x 100 mL

Shelf Life24 Months

SourcePorcine Pancreas

FormLiquid

Product TypeCell Culture Dissociation Reagent

SterilitySterile-filtered

With AdditivesPhenol Red, EDTA

Unit SizeEach

Conteúdo e armazenamento

Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 24 months from date of manufacture

Frequently asked questions (FAQs)

Do you have a non-animal-origin enzyme for cell dissociation?

Yes, the TrypLE products contain rProtease, a non-animal, trypsin-like enzyme used for the dissociation of attachment dependent cell lines. TrypLE enzyme has demonstrated the ability to dissociate cells cultured both in serum-free and serum-supplemented systems. The catalog numbers for TrypLE Select are 12563-011 (100 mL) and 12563-029 (500 mL). Some example catalog numbers for TrypLE Express include 12604-013 without Phenol red (100 mL) and 12605-010 with Phenol red (100 mL). Both formulations are also available in additional sizes. All these products are animal origin-free.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme?

All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

What are the benefits of Gibco TrypLE reagent over trypsin?

TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citações e referências (2)

Citações e referências

Abstract

Combined effects of the bisphosphonate, zoledronic acid and the aromatase inhibitor letrozole on breast cancer cells in vitro: evidence of synergistic interaction.

Authors:Neville-Webbe HL, Coleman RE, Holen I,

Journal:Br J Cancer

PubMed ID:20160726

'Aromatase inhibitors are widely used in the treatment of oestrogen receptor-positive post-menopausal breast cancer. These patients may also be receiving the bisphosphonate, zoledronic acid (ZA) to prevent bone loss or reduce skeletal morbidity in the setting of advanced disease. The potential biological interaction of these two drugs in breast cancer ... More

Isolation, cultivation and characterization of adult murine prostate stem cells.

Authors:Lukacs RU, Goldstein AS, Lawson DA, Cheng D, Witte ON,

Journal:Nat Protoc

PubMed ID:20360765

'The successful isolation and cultivation of prostate stem cells will allow us to study their unique biological properties and their application in therapeutic approaches. Here we describe step-by-step procedures on the basis of previous work in our laboratory for the harvesting of primary prostate cells from adolescent male mice by ... More