Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Número do catálogo | Tamanho da unidade | Preço (BRL) | Disponibilidade | Quantidade | |
---|---|---|---|---|---|
25530031 conhecido também como 25530-031 | 1 g | Preço 7.806,72 Seu Preço | *** | ||
25530015 | 100 mg | Preço 1.070,08 Seu Preço | *** |
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
Proteinase K remains active:
• Over a wide pH range–optimal activity between 6.5 and 9.5
• Under denaturing conditions–e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At comparatively high temperatures–optimum digestion temperature is 65°C
Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmol of Folin-positive amino acid within 1 min at 37°C using hemoglobin as a substrate.