Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:
• Over a wide pH range—optimal activity between 6.5 and 9.5 • Under denaturing conditions—e.g., in the presence of SDS or urea • In the presence of metal chelating agents—e.g., EDTA • At comparatively high temperatures—optimum digestion temperature is 65°C
Applications Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and quality testing Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definition One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within 1 min at 37°C using hemoglobin as a substrate.
Especificações
Concentration
20 mg/mL
Purity
DNase-Free, RNase-Free
For Use With (Application)
Chromatin Biology/RNA Extraction/DNA Extraction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Quantity
5 mL
Product Type
Proteinase K
Form
Liquid
Conteúdo e armazenamento
Store in freezer (-5 to -30°C).
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