KingFisher™ Flex Purification System, KingFisher with 96 PCR head

Citações e referências (36)

Thermo Scientific™

KingFisher™ Flex Purification System, KingFisher with 96 PCR head

Prepare samples efficiently from a variety of materials with Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processors.

Número do catálogo	Quantity
5400610	Each

Número do catálogo 5400610

Preço (BRL)

Quantity:

Prepare samples efficiently from a variety of materials with the Thermo Scientific™ KingFisher™ Flex Purification System, which offers highly versatile, automated magnetic-particle processing for DNA/RNA, protein or cell purification from virtually any source. Using revolutionary magnetic particle separation technology, this system provides excellent reproduciblity and quality.

Find a flexible solution for different types of sample processing needs. With 24 magnet heads, customers can raise the processing volume up to 5 mL, resulting in increased yield of the purified product. For higher throughput needs, 96 samples can be processed with 96 magnet head.

Fully automated system yields high-speed purification of nucleic acids, proteins and cells.
High throughput system processes up to 96 samples in less than 20 minutes.
Open and flexible system lets the customer use any magnetic particle-based kit to meet the application demands.
Easy-to-use Thermo Scientific™ BindIt™ Software provides instrument control, protocol creation and modification.
Ready-made protocols for different type of applications are available.

Recommended for:

Genomics and proteomics
Target identification
Veterinary assays
Biomarker discovery
Quality control
Forensics

For Laboratory Use

Especificações

Collection Efficiency of Particles> 95%

Depth (English)23.6 in.

Depth (Metric)60 cm

DescriptionKingFisher 96 plate, PCR plate

DisplaySTART / PAUSE / STOP / OK / TURNTABLE ROTATING CLOCKWISE / TURNTABLE ROTATING COUNTERCLOCKWISE / four cursor keys / LCD

For Use With (Application)Genomics and proteomics, target identification, veterinary assays, biomarker discovery, quality control, forensics

Heating TemperatureFrom +5°C above ambient temperature to +115°C

Height (English)15 in.

Height (Metric)38 cm

Magnetic Rods96 in one frame; Interchangeable magnet heads

Particle Size (Metric)ca. >1 μm

Plate Type24 or 96-well plates: KingFisher 24 Deep Well plate (200 to 5000 μL), Microtiter 96 Deep Well plate (50 to 1000 μL), KingFisher 96 plate (20 to 200 μL), PCR plate (20 to 100 μL)

QuantityEach

Weight (English)62 lb.

Weight (Metric)28 kg

Width (English)26.8 in.

Width (Metric)68 cm

Capacity96 Samples

Format96-well Plate

Plates per Deck24 or 96

Volume (Metric) Processing20 to 100 μL

Unit SizeEach

Frequently asked questions (FAQs)

Where can I find the manual for the BindIt Software?

The User Manual for the BindIt Software can be downloaded from the BindIt Software product page online, under the Documents & Downloads section. It is also included within the BindIt Software installation file under "Documentation".

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

I cannot connect my KingFisher Flex instrument to the BindIt software. How do I troubleshoot this issue?

To determine the cause of the connection issue, we recommend checking the following:
1. Check software settings:
• Make sure the instrument selected is KingFisher Flex.
• Make sure the instrument serial number was input with the dash included and no spaces (ex, "711-xxxx").
• Make sure the communication tab was set to "USB" and not "COM".
2. Check the instrument and computer:
• The KingFisher Flex instrument screen (GUI) should be set to the "Factory Protocols" tab (blue tab) for best results.
• Make sure the port function on the Maintenance Tab (last tab) is checked for "USB".
• Try using different USB cables.
• Try a different USB port on the computer.
• Check the Windows version you are using.
• Reboot the KingFisher Flex instrument and the computer.
• Make sure the driver was installed properly. This may require the help of your local IT team.

If the connection issue persists, please contact our Technical Support team at techsupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

What Uninterruptible Power Supply (UPS) do you recommend I use with the KingFisher Flex instrument?

You can use any brand of UPS as long as it is compatible with the following KingFisher Flex instrument electrical specifications:
• Mains power supply: 100–240 Vac, 50/60 Hz, nominal automatic voltage detection
• Power consumption: 175 VA max.; 55 VA standby

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

I am getting the following error message on the KingFisher Flex instrument: error message 12. What can I do to fix this error?

The “error message 12” error usually means there is something out of place on the KingFisher Flex instrument. We recommend the following to resolve the issue:
1. Check the heat block and make sure that it is not lifted up out of the base of the unit. If it is out of the base, check to see if there is anything underneath it that is preventing it from going down. If there is nothing under the heat block, it may have gotten stuck in the up position. This will require a service call.
2. Check that the tip shield is off to the right and out of the way from underneath the magnetic head. If it is stuck in the incorrect position, this may require a service call.
3. If the turntable was moved and it clicked out of place, the rectangular footprint cannot be centered below the head. Turn off the instrument and turn it back on. The instrument should re-align itself.

If the error continues to display, please contact techsupport@thermofisher.com for assistance.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

How do I use BindIt software 4.0 to load a script from my computer to the KingFisher Flex or KingFisher Duo/Duo Prime instrument?

Please follow these steps to load a script from your computer to the KingFisher Flex or KingFisher Duo/Duo Prime instrument:
1. Use the USB cable to connect the PC to the instrument.
2. Turn on the instrument.
3. Open the BindIt software and click the Connect button.
4. Select "Find New Instrument." BindIt will automatically connect to the instrument and the instrument screen will say "Computer Control".
5. Click the "Transfer" Button.
6. Click the "Upload" button.
7. Browse to find the file and double-click it.
8. In the new window that opens:
a. KingFisher Flex: you will be prompted to enter a new name – this is not necessary if the name only contains letters, digits, dash or underscore.
• If the name contains spaces, apostrophes or any other character, you should enter a new name containing only letters, digits, dash (-) or underscore (_).
• Click the "Category" pulldown to select the instrument folder you want to load the script to.
• Click OK.
b. KingFisher Duo/Duo Prime: The KingFisher Duo and Duo Prime instruments accept all characters in the filename.
• Click the "Category" pulldown to select the instrument folder you want to load the script to.
• Click OK.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

View all KingFisher™ Flex Purification System, KingFisher with 96 PCR head FAQs

Citações e referências (36)

Citações e referências

Abstract

Profiling cholinesterase adduction: a high-throughput prioritization method for organophosphate exposure samples.

Authors:Carter MD, Crow BS, Pantazides BG, Watson CM, DeCastro BR, Thomas JD, Blake TA, Johnson RC

Journal:

PubMed ID:23954929

'A high-throughput prioritization method was developed for use with a validated confirmatory method detecting organophosphorus nerve agent exposure by immunomagnetic separation high-performance liquid chromatography tandem mass spectrometry. A ballistic gradient was incorporated into this analytical method to profile unadducted butyrylcholinesterase (BChE) in clinical samples. With Zhang et al.''s Z'' factor ... More

Development and mechanism of ?-secretase modulators for Alzheimer's disease.

Authors:Crump CJ, Johnson DS, Li YM

Journal:Biochemistry

PubMed ID:23614767

'?-Secretase is an aspartyl intramembranal protease composed of presenilin, Nicastrin, Aph1, and Pen2 with 19 transmembrane domains. ?-Secretase cleaves the amyloid precursor proteins (APP) to release Aß peptides that likely play a causative role in the pathogenesis of Alzheimer's disease (AD). In addition, ?-secretase cleaves Notch and other type I ... More

Recommended mass spectrometry-based strategies to identify botulinum neurotoxin-containing samples.

Authors:Kalb SR, Baudys J, Wang D, Barr JR

Journal:

PubMed ID:25996606

'Botulinum neurotoxins (BoNTs) cause the disease called botulism, which can be lethal. BoNTs are proteins secreted by some species of clostridia and are known to cause paralysis by interfering with nerve impulse transmission. Although the human lethal dose of BoNT is not accurately known, it is estimated to be between ... More

Quantification of ricin, RCA and comparison of enzymatic activity in 18 Ricinus communis cultivars by isotope dilution mass spectrometry.

Authors:Schieltz DM, McWilliams LG, Kuklenyik Z, Prezioso SM, Carter AJ, Williamson YM, McGrath SC, Morse SA, Barr JR

Journal:

PubMed ID:25576235

'The seeds of the Ricinus communis (Castor bean) plant are the source of the economically important commodity castor oil. Castor seeds also contain the proteins ricin and R. communis agglutinin (RCA), two toxic lectins that are hazardous to human health. Radial immunodiffusion (RID) and the enzyme linked immunosorbent assay (ELISA) ... More

Using phage and yeast display to select hundreds of monoclonal antibodies: application to antigen 85, a tuberculosis biomarker.

Authors:Ferrara F, Naranjo LA, Kumar S, Gaiotto T, Mukundan H, Swanson B, Bradbury AR

Journal:PLoS One

PubMed ID:23166701

'Current diagnostic methods for tuberculosis (TB), a major global health challenge that kills nearly two million people annually, are time-consuming and inadequate. During infection a number of bacterial molecules that play a role in the infective process are released and have been proposed as biomarkers for early TB diagnosis. Antigen ... More

View all KingFisher™ Flex Purification System, KingFisher with 96 PCR head citations