The MagniSort™ Mouse F4/80 Positive Selection Kit is designed for the magnetic separation of F4/80+ cells by positive selection. It has been optimized for the isolation of F4/80+ cells from mouse bone marrow or splenocytes utilizing a biotinylated Anti-Mouse F4/80 antibody and streptavidin-coated magnetic beads. F4/80+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from F4/80+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse F4/80, clone BM8, and Anti-Mouse CD11b, clones M1/70.

Reported Application
Magnetic Cell Separation