Pierce™ Biotin 3' End DNA Labeling Kit
Pierce™ Biotin 3' End DNA Labeling Kit
Thermo Scientific™

Pierce™ Biotin 3' End DNA Labeling Kit

The Thermo Scientific Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for useLeia mais
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Número do catálogoQuantity
8981820 Reactions
Número do catálogo 89818
Preço (BRL)
3.918,50
Each
Adicionar ao carrinho
Quantity:
20 Reactions
Preço (BRL)
3.918,50
Each
Adicionar ao carrinho
The Thermo Scientific Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.

The DNA biotinylation procedure uses terminal deoxynucleotidyl transferase (TdT) to catalyze nontemplate-directed nucleotide incorporation onto the 3'-OH end of single-stranded DNA. TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3' overhangs and blunt duplexes, albeit with a lower efficiency.

Features of the Biotin 3' End DNA Labeling Kit:

  • Non-isotopic labeling eliminates the hassle of hazardous radioactive materials or difficult-to-dispose-of waste
  • 1-3 biotinylated ribonucleotides onto the 3' end of DNA strands for less interference with hybridization or sequence-specific binding of proteins
  • Biotin-labeled probes are stable for more than one year
  • 30-minute labeling procedure is fast and efficient

    The Biotin 3' End DNA Labeling Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (Biotin-11-UTP) onto the 3' end of DNA strands. This labeling strategy has the advantage of localizing the biotin to the 3' end of the probe where it will be less likely to interfere with hybridization or sequence-specific binding of proteins. Biotin-labeled DNA probes can be used to facilitate non-isotopic detection in a variety of applications including electrophoretic mobility shift assays (EMSA), Northern or Southern blots, colony hybridizations or in situ hybridizations.

    • For Research Use Only. Not for use in diagnostic procedures.
    Especificações
    No. of Reactions20
    Product LinePierce
    Product TypeDNA Labeling Kit
    Quantity20 Reactions
    FormatKit
    Labeling TargetDNA
    Unit SizeEach
    Conteúdo e armazenamento
    Sufficient For: 20 labeling reactions (each with 5 pmol DNA)
    • 1 mL 5X TdT Reaction Buffer (-20°C)
    • 5 μL Terminal Deoxynucleotidyl Tranferase (-20°C)
    • 1 μL Biotin-11-UTP (≤4°C)
    • 14 μL Unlabeled Control Oligo (-20°C)
    • 4 μL Biotin-Control Oligo (-20°C)