GeneChip™ miRNA 4.0 Array
GeneChip™ miRNA 4.0 Array
Applied Biosystems™

GeneChip™ miRNA 4.0 Array

Many diseases, including cancer, are frequently described as diseases of disordered gene expression. It is estimated that more than 30%Leia mais
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Número do catálogoNumber of Arrays
90241330 arrays
9024112 arrays
9024126 arrays
Número do catálogo 902413
Preço (BRL)
52.328,20
Each
Adicionar ao carrinho
Number of Arrays:
30 arrays
Preço (BRL)
52.328,20
Each
Adicionar ao carrinho
Many diseases, including cancer, are frequently described as diseases of disordered gene expression. It is estimated that more than 30% of protein translation of coding genes is regulated by miRNA. There is also a large amount of growing evidence suggesting miRNA interacts with long non-coding RNA in the signaling networks that regulate alternative splicing events, which impacts cellular processes such as apoptosis, proliferation, and differentiation– all of which have shown to be causative elements in diseases such as cancer.

Measuring the changes in these critical nodes of regulation is extremely important for deciphering the biological context of differentially expressed genes. This array design is a powerful tool for studying the role of small non-coding RNAs and their involvement in a broad spectrum of developmental and physiological mechanisms.

To keep pace with the discovery of new and novel miRNA, we are pleased to offer the GeneChip™ miRNA 4.0 Array to our growing catalog of miRNA arrays. This array offers updated content with the same high performance as the previous-generation array.

GeneChip miRNA 4.0 Arrays help bring you closer to biology with:
Comprehensive coverage – Designed to interrogate all mature miRNA sequences in miRBase Release 20
Easily correlate miRNA results – Analysis files contain host gene ID, predicted and validated miRNA target genes, and clustered miRNA information
Easy analysis – Analyze human, mouse, rat, or every miRNA for all species using the same array
Low sample input – Requires as little as 130 ng total RNA
Simple, fast, and free analysis solution – Coupled with Expression Console™ Software and Transcriptome Analysis Console (TAC) Software, researchers have a complete solution from data to decision-making in minutes

For Research Use Only. Not for use in diagnostic procedures.
Especificações
Product LineGeneChip
Quantity30 arrays
TypemiRNA 4.0 Array
ArraymiRNA Profiling
FormatArray Cartridge
Number of Arrays30 arrays
SpeciesAll Organisms, Human, Mouse, Rat
Unit SizeEach

Frequently asked questions (FAQs)

Where can I find the species information for your GeneChip miRNA arrays?

The species information can be found in the annotation file of the miRNA array of interest.
Please complete the following steps:
- Go to www.thermofisher.com and go to the product page of the array of interest.
- Scroll down to support files.
- Download the annotation files for the miRNA array of interest, and open in excel.
- In the file, there will be a column, species scientific name. This column will have all the species present on the array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

For GeneChip miRNA arrays, are External RNA Controls Consortium (ERCC) controls tiled on the array?

The ERCC controls are not tiled onto GeneChip miRNA arrays because they would not work with the miRNA array/assay system. The FlashTag Biotin HSR RNA Labeling Kits used to label miRNAs is optimized for shortRNAs. Even though longer mRNAs like the ERCC controls will be labeled, the HWS (hyb/wash/stain) conditions are not optimal and consequently, the longer mRNAs typically do not get detected.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Which of the gene arrays are compatible with the miRNA interaction network in Transcriptome Analysis Console (TAC) Software?

The miRNA interaction network in TAC Software is available for all gene arrays, including model organisms. To have the interaction network available, the same samples must have been processed through both the gene array and the miRNA array. Additionally, the gene array being used must have a gene symbol column displayed in their annotation.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Does GeneChip miRNA 4.0 Array contain any feline miRNA content?

No, GeneChip miRNA 4.0 Array does not contain any microRNAs specific to felines.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How are sno/sca and hairpin probe sets selected and how does that impact my signal summarization?

Probes for snoRNA, scaRNA, and hairpins are selected to maximize probe response to target concentration in the sample while minimizing cross-hybridization to other potential targets in the sample. In order to minimize cross-hybridization, potential targets are inferred from the landscape of known sequences from the input dataset and used in a filtering process called pruning which penalizes probe candidates that may cross-hybridize to unwanted targets. As the landscape of known sequences improves, we can improve our pruning set to avoid probe candidates previously thought to be unique. An improved pruning set will reduce the chances a probe will cross-hybridize to unwanted target, improving the probe set representation of the intended target. As a result of the improved probe selection, few probe sets are required to represent the same number of sequences.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.