ACK Lysing Buffer

ACK (Ammonium-Chloride-Potassium) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, suchLeia mais

Número do catálogo	Quantity
A1049201	100 mL

Número do catálogo A1049201

Preço (BRL)

175,60

Adicionar ao carrinho

Quantity:

100 mL

Preço (BRL)

175,60

Adicionar ao carrinho

ACK (Ammonium-Chloride-Potassium) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. The complete formulation and lysis protocol are available in our Technical Reference Library.

cGMP manufacturing and quality system
ACK Lysing Buffer is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

For Research Use Only. Not for use in diagnostic procedures.

Especificações

Chemical Name or MaterialLysing Buffer

Recommended StorageStorage conditions: 15–30°C. Protect from light.
Shipping conditions: ambient
Shelf life: 12 months from date of manufacture

Shelf Life12 Months

SterilitySterile

Physical FormLiquid

Product LineGibco

Quantity100 mL

Unit SizeEach

Frequently asked questions (FAQs)

What alternative do you offer for ACK Lysing Buffer (Cat. No. A1049201)?

Alternative products we offer are eBioscience 1X RBC Lysis Buffer (Cat. No. 00-4333-57) or eBioscience 10X RBC Lysis Buffer (Multi-species) (Cat. No. 00-4300-54).

Can I use ACK Lysing Buffer with heparinized blood?

Yes, ACK Lysing Buffer can be used with heparinized blood. The principle of lysis is osmotic treatment.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I use ACK Lysing Buffer to lyse red blood cells?

A volume of ACK lysing buffer corresponding to 10-20 times the volume of the blood sample is sufficient to lyse red blood cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citações e referências (1)

Citações e referências

Abstract

Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.

Authors:Avci FY, Li X, Tsuji M, Kasper DL,

Journal:Nat Protoc

PubMed ID:23196974

Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized ... More