Human Mammary Epithelial Cells (HMEC)
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Human Mammary Epithelial Cells (HMEC)

Primary human mammary epithelial cells, cryopreserved at the end of the 6th culture.• Viability at least 70%• ≥500,000 viable cells/vial•Leia mais
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Número do catálogoQuantity
A105651 vial
Número do catálogo A10565
Preço (BRL)
-
Quantity:
1 vial
Primary human mammary epithelial cells, cryopreserved at the end of the 6th culture.

• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
• Positive for Cytokeratins 5⁄6, 8, 18, and E-cadherin
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Donor SourceSingle Donor
No. of Cells≥500000
Product LineCascade Biologics
Product TypeHuman Mammary Epithelial Cells
Quantity1 vial
Shipping ConditionDry Ice
Tested ForHepatitis B (HBV), Hepatitis C (HCV), Sterility Testing (Bacteria & Fungi), Mycoplasma, Growth Performance Assay, HIV-1
AgeAdult
Cell TypeEpithelial Cells (Mammary)
Culture TypeAdherent Cell Culture
FormCryopreserved
SpeciesHuman
Unit SizeEach
Conteúdo e armazenamento

Liquid nitrogen temperature

Frequently asked questions (FAQs)

Is it difficult to culture/passage Human Mammary Epithelial Cells (HMEC)?

No. HMEC cultures are easily initiated at 2.5 x 10E3 cells per cm2 in standard tissue culture plasticware and passaged using a trypsin/EDTA solution.

How many T-25 flasks can I seed from 1 vial of Human Mammary Epithelial Cells (HMEC)?

One vial (500,000 viable cells) can seed eight T-25 flasks at 2,500 viable cells per cm2. A hemocytometer should be used to accurately count cells and to help ensure an appropriate seeding density.

What is the difference between M171 and HuMEC growth media?

Both media are designed for culture of HMEC. However, in-house studies show HuMEC Ready Medium (Thermo Fisher Scientific Cat. No. 12752-010) outperforms M171/MEGS (Thermo Fisher Scientific Cat. No. M-171-500/S-015-5) in both growth rate and lifespan measurements of HMEC. There is no difference between media when staining for cell-specific markers using indirect ICC.

How does passage number correlate with population doublings for Human Mammary Epithelial Cells (HMEC)?

One passage is equivalent to approximately 4 population doublings when using the recommended subculture conditions.

What are typical doubling times for Human Mammary Epithelial Cells (HMEC)?

HMEC usually have population doubling times of 24-30 hr during the guaranteed lifespan.

View all Human Mammary Epithelial Cells (HMEC) FAQs