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Invitrogen™
GeneArt™ Linear pUC19L Vector for Seamless Cloning
Número do catálogo: A13289
Invitrogen™
GeneArt™ Linear pUC19L Vector for Seamless Cloning
Número do catálogo: A13289
| Número do catálogo | Tamanho da unidade | Preço (BRL) | |
|---|---|---|---|
| A13289 | 20 reactions | Entre em contato › |
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The GeneArt™ Linear pUC19L Vector is a ready-to-use, linear version of the popular cloning vector pUC19. The pUC19L plasmid (2.7 Kbp), is specifically designed as a cloning and DNA assembly vector for the GeneArt™ Seamless Cloning and Assembly Kit (cat # A13288). The vector is manufactured by digesting supercoiled pUC19 plasmid with the restriction enzymes PstI and KpnI, and tested for cloning of up to 4 DNA fragments.
Some of the key features and elements of the pUC19L vector include:
• lacZα gene allows easy blue⁄white colony screening for selection of recombinants
• pUC origin of replication (high copy vector)
• Ampicillin resistance for selection in E. coli
• 6 convenient and validated restriction sites flanking the cloning region
• Tested for functionality by assembly with 4 DNA fragments using the GeneArt™ Seamless Cloning and Assembly Kit
Some of the key features and elements of the pUC19L vector include:
• lacZα gene allows easy blue⁄white colony screening for selection of recombinants
• pUC origin of replication (high copy vector)
• Ampicillin resistance for selection in E. coli
• 6 convenient and validated restriction sites flanking the cloning region
• Tested for functionality by assembly with 4 DNA fragments using the GeneArt™ Seamless Cloning and Assembly Kit
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Product Type
Cloning Vector
Reporter Gene
Beta-Gal (lacZ)
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Replication Origin
pUC Origin
Shipping Condition
Dry Ice
Size Range
13 kb total (vector plus all inserts)
Cloning Method
Seamless Cloning
Concentration
50 ng/mL
Quantity
Up to 4 Fragments
Vector
Seamless Cloning Vectors
Product Line
GeneArt™
Conteúdo e armazenamento
The product contains 5 vials of linear pUC19L vector at a concentration of 50ng⁄ml. Each vial contains enough plasmid for 4 cloning reactions using the GeneArt™ Seamless Cloning and Assembly Kit (A13288 - must be purchased separately).
Store the product at -80°C.
The vector is guaranteed stable for 6 months when properly stored.
Store the product at -80°C.
The vector is guaranteed stable for 6 months when properly stored.
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