QuantStudio™ 5 Real-Time PCR System, 96-well, 0.2 mL, laptop
QuantStudio™ 5 Real-Time PCR System, 96-well, 0.2 mL, laptop
Applied Biosystems™

QuantStudio™ 5 Real-Time PCR System, 96-well, 0.2 mL, laptop

The Applied Biosystems QuantStudio 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, andLeia mais
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Número do catálogoQuantity
A285691 system
Número do catálogo A28569
Preço (BRL)
-
Quantity:
1 system
The Applied Biosystems QuantStudio 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to iConnect, Thermo Fisher's cloud-based platform, the QuantStudio 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex technology (featuring six decoupled channels and white LED) and featuring six independent Veriflex temperature zones, the QuantStudio 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

Quantstudio 3/5 Firmware and Design & Analysis Software >

Features of the QuantStudio 5 system with 96-well block include:

• Proven OptiFlex technology (six decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2 mL format or 96-well 0.1 mL format

iConnect
iConnect provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within iConnect are web browser-based software tools that are flexible, fast and easy to use.

Easy to use
The QuantStudio 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update

Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows 10 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio 5 system offers users greater control of experiment data

Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology. In the 96-well system this technology features six decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Display TypeTouchscreen
For Use With (Equipment)QuantStudio™
Format96-well Plate
Product LineQuantStudio
Quantity1 system
Shipping ConditionRoom Temperature
Volume (Metric) Thermal Block Sample0.2 ml
Block FormatNon-interchangeable
Unit SizeEach
Conteúdo e armazenamento
Laptop Computer

Frequently asked questions (FAQs)

What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

I am unable to download the current software update for the QuantStudio 5 Real-Time PCR System from the Thermo Fisher website. Why?

We recommend trying different browsers (Google Chrome, Firefox, and Microsoft Edge) and/or removing cookies from your browser. If this does not resolve the issue, please contact Technical Support at techsupport@thermofisher.com.

What is the admin pin for QuantStudio 3 and 5 Real-Time PCR Systems?

If the admin PIN has not been changed, 1234 should work. If it does not work, you can also try 0000.
However, if the admin PIN has been changed and none of the above PINs work, follow the instructions in the QuantStudio 3 and 5 Real-Time PCR Systems manual, page 100.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Is the black frame removable from the QuantStudio 3 and 5 Real-Time PCR Systems 96 well, 0.2 ml block?

We do not recommend physically removing the black frame. It is necessary for the plates to fit properly and should stay on the instrument. If it has been accidently removed or lost, please contact ICS to replace the spare part at (800) 9550-6288 options 3-2 or email technicalhardwaresupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

Citações e referências (3128)

Citações e referências
Abstract
A novel HIF2A mutation causes dyslipidemia and promotes hepatic lipid accumulation.
Authors:Gao F,Yao Q,Zhu J,Chen W,Feng X,Feng B,Wu J,Pacak K,Rosenblum J,Yu J,Zhuang Z,Cao H,Li L
Journal:Pharmacological research
PubMed ID:37453673
Hypoxia-inducible factor-2α (HIF-2α) is a transcription factor responsible for regulating genes related to angiogenesis and metabolism. This study aims to explore the effect of a previously unreported mutation c.C2473T (p.R825S) in the C-terminal transactivation domain (CTAD) of HIF-2α that we detected in tissue of patients with liver disease. We sequenced ... More
Profiling of oxBS-450K 5-hydroxymethylcytosine in human placenta and brain reveals enrichment at imprinted loci.
Authors:Hernandez Mora JR,Sanchez-Delgado M,Petazzi P,Moran S,Esteller M,Iglesias-Platas I,Monk D
Journal:Epigenetics
PubMed ID:28678681
DNA methylation (5-methylcytosine, 5 mC) is involved in many cellular processes and is an epigenetic mechanism primarily associated with transcriptional repression. The recent discovery that 5 mC can be oxidized to 5-hydromethylcytosine (5hmC) by TET proteins has revealed the “sixth base” of DNA and provides additional complexity to what was originally thought ... More
Molecular Evolution and Epidemiological Characteristics of SARS COV-2 in (Northwestern) Poland.
Authors:Serwin K,Ossowski A,Szargut M,Cytacka S,Urbańska A,Majchrzak A,Niedźwiedź A,Czerska E,Pawińska-Matecka A,Gołąb J,Parczewski M
Journal:Viruses
PubMed ID:34372500
The emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) evolved into a worldwide outbreak, with the first Polish cases in February/March 2020. This study aimed to investigate the molecular epidemiology of the circulating virus lineages between March 2020 and February 2021. We performed variant identification, spike mutation pattern analysis, and ... More
Bacteria and protozoa with pathogenic potential in Ixodes ricinus ticks in Viennese recreational areas.
Authors:Schötta AM,Stelzer T,Stanek G,Stockinger H,Wijnveld M
Journal:Wiener klinische Wochenschrift
PubMed ID:35689113
Ixodes ricinus is the most relevant vector for tick-borne diseases in Austria and responsible for the transmission of Borrelia burgdorferi sensu lato (s. l.), which causes Lyme borreliosis in humans; however, also other bacteria and protozoa can be found in ticks and have the potential of infecting people and animals. In ... More
β-Catenin/Lin28/let-7 regulatory network determines type II alveolar epithelial stem cell differentiation phenotypes following thoracic irradiation.
Authors:Liu X,Zhang T,Zhou J,Xiao Z,Li Y,Zhang Y,Yue H,Li Z,Tian J
Journal:Journal of radiation research
PubMed ID:33302295
The contribution of type II alveolar epithelial stem cells (AEC II) to radiation-induced lung fibrosis (RILF) is largely unknown. Cell differentiation phenotypes are determined by the balance between Lin28 and lethal-7 microRNA (let-7 miRNA). Lin28 is activated by β-catenin. The aim of this study was to track AEC II phenotypes ... More