Oncomine™ Focus Assay, AmpliSeq™ Library

The Oncomine Focus Assay is a targeted next-generation sequencing (NGS), multi-biomarker assay that enables the detection of variants across 52 cancer-relevant genes from DNA and RNA in a single workflow. The assay provides reagents for library construction and two pools - one DNA and one RNA - of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Using as little as 10 ng of nucleic acid per primer pool, the assay enables analysis of hotpots, SNVs, indels, CNVs, and gene fusions in a single sequencing run.

The Oncomine Focus Assay, AmpliSeq Library, is for use as part of the Ion Chef and Ion GeneStudio S5 Systems workflow. Scalability and flexibility are achieved using Ion Xpress or IonCode barcode adapters for multiplexing barcoded samples on Ion S5 chips. For a kit that also includes reagents and consumables for automated library preparation on the Ion Chef System, please see the Oncomine Focus Assay, Chef-Ready Library.

From sample to answer

The Oncomine Focus Library Assay is part of an NGS workflow that helps researchers filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

Learn more about Oncomine Focus Assay and Oncomine oncology.