Ion GeneStudio™ S5 Plus System

The Ion GeneStudio™ S5 Plus System is a semiconductor-based next-generation sequencing (NGS) system that enables simple, flexible targeted sequencing workflows for clinical research and research laboratories. From targeted gene panels to exomes and transcriptomes, the Ion GeneStudio S5 Plus System helps laboratories manage both small and high-throughput sequencing projects across oncology, inherited disease, reproductive health, infectious disease, microbial genomics, and transcriptomics applications.

With cartridge-based reagents and the flexible Ion 6-Series chip portfolio, including the new high-capacity Ion 560™ Chip, the system is simple to use while providing scalable sequencing throughput. The Ion GeneStudio S5 Plus System supports a broad range of targeted DNA sequencing and RNA sequencing applications, enabling laboratories to increase sample throughput or sequencing depth using the same proven workflow.

The Ion GeneStudio™ S5 Plus system has intermediate computing capacity between the Ion GeneStudio S5 (base model) and the Ion GeneStudio S5 Prime systems. It supports all 6-Series Chips, from the 510 up to the 560 Chip, but it can only sequence 1 550 or 560 Chip per day, different from the Ion GeneStudio Prime system that can support up to 2 550 or 560 Chips per day.

Key features:

• Select the chip (Ion 510 - 560 Chip) that fits your application and throughput needs to achieve from 2–280 million reads per run
• Expand sequencing capacity with the Ion 560 Chip to support more samples per run or deeper sequencing coverage for compatible targeted sequencing workflows
• Reduce setup time and complexity with cartridge-based plug-and-play reagents
• Complete sequencing and primary analysis in hours to move experiments forward faster

The Ion GeneStudio™ S5 Series

The Ion GeneStudio™ S5 systems share the same foundational semiconductor sequencing technology and fluidic architecture across all three instruments: the Ion GeneStudio™ S5 system, the Ion GeneStudio™ S5 Plus system, and the Ion GeneStudio™ S5 Prime system. The primary difference across the three instruments is on-sequencer analysis speed and supported chips. While the GeneStudio™ S5 Plus and Prime systems can run any of the Chips, from the 510 to the 560, the GeneStudio™ base model can only support the 510, 520, 530 and 540 Chips.

Speed

The Ion GeneStudio S5 Series leverages the speed of semiconductor sequencing to produce high-quality sequencing data in just a few hours. When paired with the Ion Chef™ System, laboratories can go from library preparation to sequencing data in as little as 24 hours with approximately 45 minutes of total hands-on time.

Simplicity

With single-day installation, a straightforward user interface, and cartridge-based reagents, the Ion GeneStudio S5 systems are easy to use benchtop sequencers. Combined with Ion AmpliSeq™ technology for targeted panel design, the Ion Chef™ System for automated library and template preparation, and Ion Reporter™ Software for automated variant annotation, targeted NGS workflows are simpler than ever.

Small sample input

Low-input DNA and RNA from challenging sample types—including formalin-fixed paraffin-embedded (FFPE) tissue, fine needle aspirates, and circulating cell-free DNA and RNA (cfDNA/cfRNA)—can be difficult to sequence on other next-generation sequencing platforms. With Ion AmpliSeq technology and the Ion GeneStudio S5 Series, laboratories can sequence with as little as 1 ng of DNA or RNA input.

Scalability

Why purchase multiple sequencing systems when one platform can support all your targeted sequencing needs? The Ion 6 Series chip portfolio—including the new Ion 560 Chip—provides multiple throughput options so laboratories can efficiently scale from small research studies to high-volume targeted sequencing projects. Choose the sequencing output that best matches your application, from small targeted panels to comprehensive genomic profiling (CGP), transcriptomics, and custom AmpliSeq™ workflows, while maintaining the same simple workflow.

Simple analysis solutions

Torrent Suite™ Software and Ion Reporter™ Software make NGS data analysis accessible for both new and experienced users. Plan, monitor, track, and analyze sequencing runs using Torrent Suite Software (pre-installed on the Sequencer). Integrate, annotate, and interpret variants using Ion Reporter Software, available as either a cloud-based or local deployment. LIMS connectivity services, including API training and implementation support, help laboratories integrate sequencing workflows into existing laboratory information systems.

Services and support

With more than 2,000 field personnel worldwide, Thermo Fisher Scientific provides one of the industry's largest global networks of Field Application Specialists and Service Engineers to support your laboratory throughout implementation and routine operation.

• Service plans:—Instrument service agreements designed to support routine operation and system availability.
• Qualification services:—Installation and operational qualification services that document instrument performance according to manufacturer specifications.
• Training:—Instructor-led and virtual courses covering instrument operation, workflow setup, troubleshooting, and NGS applications.
• Bioinformatics support:—Resources for workflow implementation, software configuration, data analysis, and downstream applications.