Ammonium Acetate (5 M), RNase-free
Ammonium Acetate (5 M), RNase-free
Invitrogen™

Ammonium Acetate (5 M), RNase-free

Ambion Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 500 mL. The solution isLeia mais
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Número do catálogoQuantity
AM9070G100 mL
AM9071500 mL
Número do catálogo AM9070G
Preço (BRL)
-
Quantity:
100 mL
Ambion Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 500 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion's nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Chemical Name or MaterialAmmonium Acetate
Packaging TypeBottle
Product LineAmbion
PurityMolecular Biology Grade
Quantity100 mL
Shipping ConditionRoom Temperature
Concentration5 M
FormLiquid
Unit SizeEach
Conteúdo e armazenamento
Store at room temperature.

Frequently asked questions (FAQs)

What salts can be used to precipitate total RNA? What are the advantages of each? Do they require ethanol?

The three salts that can be used are:

- Guanidine thiocyanate, which requires ethanol. Guanidine thiocyanate is a common agent used for isolating RNA and we recommend it especially for tissues high in ribonuclease activity, such as the pancreas or spleen.
- Ammonium acetate, which requires ethanol. Ammonium acetate is useful when reducing coprecipitation of unwanted dNTPs and oligosaccharides. However, it should not be used when the nucleic acid will be phosphorylated using T4 polynucleotide kinase, since this enzyme is inhibited by ammonium ions.
- Lithium chloride, which does not require ethanol. LiCl is very effective in precipitating RNA but does not efficiently precipitate protein or DNA. It also does not precipitate unincorporated nucleotides.