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Citações e referências (84)
Invitrogen™
BCECF Acid (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein), mixed isomers
BCECF is ideal for measuring changes ± 1 pH unit around pH 7, because it is a dual-excitation ratiometric pHLeia mais
| Número do catálogo | Quantity |
|---|---|
| B1151 conhecido também como B-1151 | 1 mg |
Número do catálogo B1151
conhecido também como B-1151
Preço (BRL)
-
Quantity:
1 mg
BCECF is ideal for measuring changes ± 1 pH unit around pH 7, because it is a dual-excitation ratiometric pH indicator with a pKa of ∼6.98. pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ∼490 nm versus the emission intensity when excited at its isobestic point of ∼440 nm.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Detection MethodFluorescence
Dye TypepH Indicator
Quantity1 mg
Shipping ConditionRoom Temperature
For Use With (Equipment)Microplate Reader
Product TypepH indicator
Unit SizeEach
Conteúdo e armazenamento
Store at room temperature and protect from light.
Citações e referências (84)
Citações e referências
Abstract
Measurement of intracellular pH.
Journal:Methods Cell Biol
PubMed ID:7532259
Fluorescent indicators of ion concentrations.
Journal:Methods Cell Biol
PubMed ID:2538708
Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
Journal:Biochemistry
PubMed ID:7849020
'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent
Luminal pH regulated calcium release kinetics in sarcoplasmic reticulum vesicles.
Journal:Biochemistry
PubMed ID:8873610
'Calcium binding to triads isolated from rabbit skeletal muscle followed a single hyperbolic function in the pH range 5.5-8.0. Maximal binding was obtained at pH 8.0; decreasing the pH decreased the binding capacity and, at pH < or = 6.0, increased Kd 2-fold. These results indicate that lowering the pH
Modulation of the hyperpolarization-activated cation current of rat thalamic relay neurones by intracellular pH.
Journal:J Physiol
PubMed ID:10457065
'1. Properties of the hyperpolarization-activated cation current (Ih) were investigated in thalamocortical neurones of an in vitro slice preparation of the rat ventrobasal thalamic complex (VB) before and during changes of pipette pH (pHp), intracellular pH (pHi) and bath pH (pHb) using the whole-cell patch-clamp technique and fluorescence ratio imaging