BacLight™ RedoxSensor™ Green Vitality Kit

<i>Bac</i>Light™ RedoxSensor™ Green Vitality Kit

Citações e referências (8)

Invitrogen™

BacLight™ RedoxSensor™ Green Vitality Kit

The RedoxSensor™ Green reagent included in the BacLight™ RedoxSensor™ Green Vitality Kit is an indicator of bacterial reductase activity. ThisLeia mais

Número do catálogo	Quantity
B34954	200 kit

Número do catálogo B34954

Preço (BRL)

4.469,47

Adicionar ao carrinho

Quantity:

200 kit

Preço (BRL)

4.469,47

Adicionar ao carrinho

The RedoxSensor™ Green reagent included in the BacLight™ RedoxSensor™ Green Vitality Kit is an indicator of bacterial reductase activity. This reductase activity is, in turn, a reliable marker for changes in electron transport chain function and for changes in vitality that occurs following antibiotic treatment. RedoxSensor™ Green reagent penetrates both gram-positive and gram-negative bacteria. Following reduction, the RedoxSensor™ Green reagent will produce a stable green-fluorescent signal in 10 minutes that is compatible with formaldehyde fixation techniques.

View additional information about all microbiology assays for flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.

Especificações

Cell TypeBacteria

Detection MethodFluorescence

Dye TypeRedoxSensor™ green, PI

FormSolution

FormatTube(s), Slide(s)

Quantity200 kit

Shipping ConditionRoom Temperature

SolubilityDMSO (Dimethylsulfoxide)

EmissionPI: 490/635, RedoxSensor™ green: 490/520

For Use With (Equipment)Fluorescence Microscope, Flow Cytometer

Product LineBacLight, RedoxSensor

Product TypeGreen Vitality Kit

Unit SizeEach

Conteúdo e armazenamento

Contains 1 vial of RedoxSensor™ green (200 μL, 1 mM solution in DMSO), 1 vial of propidium iodide (300 μL, 20 mM solution in DMSO), 1 vial of sodium azide (1 mL of a 2 M solution in water), and 1 vial of CCCP (400 μL, 5mM in DMSO).

Frequently asked questions (FAQs)

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the excitation/emission of the RedoxSensor Green reagent?

The excitation/emission of the RedoxSensor Green reagent, in reduced form, is 490/520 nm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citações e referências (8)

Citações e referências

Abstract

Respiration response imaging for real-time detection of microbial function at the single-cell level.

Authors:Konopka MC, Strovas TJ, Ojala DS, Chistoserdova L, Lidstrom ME, Kalyuzhnaya MG,

Journal:Appl Environ Microbiol

PubMed ID:21075887

'The ability to detect specific functions of uncultured microbial cells in complex natural communities remains one of the most difficult tasks of environmental microbiology. Here we present respiration response imaging (RRI) as a novel fluorescence microscopy-based approach for the identification of microbial function, such as the ability to use C(1) ... More

Extracellular reduction of uranium via Geobacter conductive pili as a protective cellular mechanism.

Authors:Cologgi DL, Lampa-Pastirk S, Speers AM, Kelly SD, Reguera G,

Journal:Proc Natl Acad Sci U S A

PubMed ID:21896750

'The in situ stimulation of Fe(III) oxide reduction by Geobacter bacteria leads to the concomitant precipitation of hexavalent uranium [U(VI)] from groundwater. Despite its promise for the bioremediation of uranium contaminants, the biological mechanism behind this reaction remains elusive. Because Fe(III) oxide reduction requires the expression of Geobacter''s conductive pili, ... More

Fullerene water suspension (nC60) exerts antibacterial effects via ROS-independent protein oxidation.

Authors:Lyon DY, Alvarez PJ,

Journal:Environ Sci Technol

PubMed ID:19031913

Buckminsterfullerene (C60) can form water suspensions (nC60) that exert toxic effects. While reactive oxygen species (ROS) generation has been implicated as the mechanism for mammalian cytotoxicity, we propose that nC60 exerts ROS-independent oxidative stress in bacteria, with evidence of protein oxidation, changes in cell membrane potential, and interruption of cellular ... More

Oxygen consumption rates of bacteria under nutrient-limited conditions.

Authors:Riedel TE, Berelson WM, Nealson KH, Finkel SE,

Journal:

PubMed ID:23770901

Many environments on Earth experience nutrient limitation and as a result have nongrowing or very slowly growing bacterial populations. To better understand bacterial respiration under environmentally relevant conditions, the effect of nutrient limitation on respiration rates of heterotrophic bacteria was measured. The oxygen consumption and population density of batch cultures ... More

In vitro susceptibility and cellular uptake for a new class of antimicrobial agents: dinuclear ruthenium(II) complexes.

Authors:Li F, Feterl M, Mulyana Y, Warner JM, Collins JG, Keene FR,

Journal:J Antimicrob Chemother

PubMed ID:22865383

To determine the in vitro susceptibility and cellular uptake for a series of dinuclear ruthenium(II) complexes [{Ru(phen)(2)}(2){µ-bb(n)}](4+) (Rubb(n)), and the mononuclear complexes [Ru(Me(4)phen)(3)](2+) and [Ru(phen)(2)(bb(7))](2+) against Staphylococcus aureus, methicillin-resistant S. aureus, Escherichia coli and Pseudomonas aeruginosa. The in vitro susceptibility was determined by MIC and MBC assays, and time-kill curve ... More

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