Orbitrap™ Ascend Editions Tribrid™ Mass Spectrometer

Thermo Scientific™

Orbitrap™ Ascend Editions Tribrid™ Mass Spectrometer

Name: Orbitrap™ Ascend Editions Tribrid™ Mass Spectrometer
SKU: B51003848

Scale up your science for proteomics and native protein analysis with versatility and ease-of-use with Orbitrap Ascend Editions Tribrid mass spectrometers.

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Número do catálogo	Model
B51003848	Orbitrap Ascend Structural Biology Tribrid MS
FSN06-10000	Orbitrap Ascend Tribrid MS
B51003847	Orbitrap Ascend MultiOmics Tribrid MS
B51003849	Orbitrap Ascend BioPharma Tribrid MS

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Número do catálogo B51003848

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Model:

Orbitrap Ascend Structural Biology Tribrid MS

Exceed experimental expectation and versatility with recommended MultiOmics, Structural Biology, and BioPharma configurations of the Thermo Scientific™ Orbitrap Ascend Editions Tribrid™ Mass Spectrometer. Each Edition provides a tailored configuration of options for each application space, simplifying your decisions and streamlining your research. Characterize even the largest biopharmaceuticals and protein complexes with the Native MS option, which extends the Orbitrap mass range up to m/z 16,000 and new quadrupole isolation up to m/z 8,000. Unlock the potential of alternate fragmentation techniques to decipher the most challenging molecules.

With three unique fit-for-purpose Editions that are tailored for MultiOmics; Structural Biology; and BioPharma application areas, Orbitrap Ascend Editions Tribrid mass spectrometers ensure high-quality data acquisition. Whether you're focused on multiplexed quantitative proteomics, metabolomics, lipidomics or glycomics, small molecule-based pharmaceuticals or biotherapeutics, single-cell or deciphering higher-order protein structures; these instruments are uniquely suited to your needs.

Native MS option: Orbitrap extended mass range up to m/z 16000 and new quadrupole isolation up to m/z 8000 for improved selectivity and MSⁿ sensitivity of macromolecules.
PTCR option: Reduce m/z spectrum complexity to enhance proteoform characterization at MS¹ and MS² levels.
UVPD option: Improved fragmentation for top-down proteomics, oligonucleotides, lipidomics, and other analytes
Easy-ETD option: Achieve high sequence coverage of proteins, including site localization of labile PTMs.
EASY-IC ion source option: Obtain sub-1ppm mass accuracy with internal calibration.
Dual IRMs: Confidently identify more molecules with two ion-routing multiples to increase scan rate and sensitivity.
Real-Time Library Search: Provides higher confidence identifications for peptide or small molecule analysis using MSⁿ.
Auto-Ready ion source: Spend less time on instrument setup with a new dedicated source for autocalibration.
Pre-built method templates: Optimized methods for each Edition system, covering diverse applications.
Dry-Pump: Environmentally friendly, oil-free dry pump is standard to decrease maintenance while providing leak-free vacuum performance.

Especificações

Dimensions (D x W x H)35 x 50 x 28 in. (81 x 127 x 70 cm)

Dynamic Range> 5000 within a single scan

Mass Accuracy< 3 ppm RMS using external calibration

ModelOrbitrap Ascend Structural Biology Tribrid MS

Polarity SwitchingOne full experimental cycle acquired in < 1.1 seconds

Scan RatesUp to 45 Hz

Weight (Metric)181 kg (400 lb)

Mass Range40 to 6000 m/z, recommended options: up to 16,000 m/z)

Max. ResolutionUp to 480,000 at m/z 200

TypeTribrid MS IC/ETD, PTCR,Native

Unit SizeEach

Frequently asked questions (FAQs)

For labs focused on sensitivity, low-input samples, or PTM/complex proteomics, what performance improvements can they expect with Orbitrap Ascend Tribrid MS over previous instruments?

The Orbitrap Ascend Tribrid MS achieves ~20% higher phosphoproteome coverage compared to the previous Orbitrap Eclipse Tribrid MS (under matched conditions) and showed ~25% improvement in PTM site localization accuracy. The improved ion optics and increased acquisition rates translate directly into more identifications per unit time—for example, in a TMTpro 18-plex experiment the Orbitrap Ascend Tribrid quantified >8,000 proteins in less than 9 hours (a ~30% reduction in time compared to the previous instrument). For low-input workflows, the improved ion transmission and flexibility in fragmentation make the system especially well positioned for challenging samples.

What advanced acquisition modes or intelligent software features are standard to the Orbitrap Ascend Tribrid MS that improve productivity and data quality?

The Orbitrap Ascend Tribrid MS includes intelligent acquisition and control features such as Real-Time Search (RTS) and Real-Time Library Search (RTLS) to trigger MS/MS or MSⁿ events based on library matches or spectral patterns. It also offers method templates optimized for workflows (called “pre-built optimized templates”) and the Auto-Ready ion source for automated calibration without manual intervention. These features help reduce setup time, improve reproducibility, and offer higher throughput and reliability than many older systems.

Why would I choose an Orbitrap Ascend Tribrid MS for multi-omics and biopharmaceutical workflows over specialized single-domain systems?

The MultiOmics, BioPharma, and Structural Biology Editions of the Thermo Scientific Orbitrap Ascend Tribrid Mass Spectrometer provide tailored hardware and software to the unique application space. For example, to enable native protein complex and biotherapeutic characterization, the Native MS option supports mass detection up to m/z 16,000 and quadrupole isolation up to m/z 8,000. At the same time, for multiomics (metabolomics, lipidomics, proteomics) it supports high-throughput multiplexed TMT workflows (e.g., TMTpro 32-plex) and advanced fragmentation, including ETD and UVPD.

What are the main architecture and performance advantages of the Orbitrap Ascend Tribrid MS compared to earlier Tribrid or HRAM systems?

The Orbitrap Ascend Tribrid MS features a unique Tribrid architecture (quadrupole → dual-pressure linear ion trap → Orbitrap mass analyzer) and incorporates several hardware innovations: a high-capacity transfer tube and modified electrodynamic ion funnel for increased ion flux; a segmented “QR5” quadrupole with hyperbolic surfaces for improved ion transmission; dual Ion Routing Multipoles for better capture and dissociation of ions.

Compared with prior-generation Tribrid systems, the Orbitrap Ascend Trbrid delivers higher acquisition rates (e.g., up to ~45 Hz at 7,500 resolution in the Orbitrap; up to ~50 Hz in the ion trap) and improved sensitivity for labile compounds. These capabilities make it more versatile and higher-performing than many conventional HRAM or single-analyzer systems.