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Citações e referências (382)
Invitrogen™
6-FAM (6-Carboxyfluorescein), single isomer
The single isomer, 6-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activationLeia mais
| Número do catálogo | Quantity |
|---|---|
| C1360 | 100 mg |
Número do catálogo C1360
Preço (BRL)
1.860,56
Each
Quantity:
100 mg
Preço (BRL)
1.860,56
Each
The single isomer, 6-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Chemical ReactivityAmine
Excitation488
Label or DyeFITC (Fluorescein)
Product Type6-FAM
Quantity100 mg
Reactive MoietyCarboxylic Acid
Shipping ConditionRoom Temperature
Label TypeClassic Dyes
Unit SizeEach
Conteúdo e armazenamento
Store at room temperature and protect from light.
Citações e referências (382)
Citações e referências
Abstract
Membrane vesicles containing the Sendai virus binding glycoprotein, but not the viral fusion protein, fuse with phosphatidylserine liposomes at low pH.
Journal:Biochemistry
PubMed ID:3021204
Membrane vesicles containing the Sendai virus hemagglutinin/neuraminidase (HN) glycoprotein were able to induce carboxyfluorescein (CF) release from loaded phosphatidylserine (PS) but not loaded phosphatidylcholine (PC) liposomes. Similarly, fluorescence dequenching was observed only when HN vesicles, bearing self-quenched N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine (N-NBD-PE), were incubated with PS but not PC liposomes. Thus, fusion between
Receptor-mediated endocytosis of antibody-opsonized liposomes by tumor cells.
Journal:Proc Natl Acad Sci U S A
PubMed ID:7001454
'Specific receptor-mediated delivery of the contents of small, sonicated liposomes was studied with three murine tumor cell types: an IgG Fc receptor-negative nonphagocytic line (EL4); an Fc receptor-positive phagocytic line (P388D1); and an Fc receptor-positive nonphagocytic line (P388). The liposomes (formed from phosphatidylcholines, cholesterol, and dinitrophenyl-substituted phosphatidylethanolamine) contained carboxyfluorescein as
Reconstitution of functional water channels in liposomes containing purified red cell CHIP28 protein.
Journal:Biochemistry
PubMed ID:1510932
'Water rapidly crosses the plasma membranes of red blood cells (RBCs) and renal tubules through highly specialized channels. CHIP28 is an abundant integral membrane protein in RBCs and renal tubules, and Xenopus laevis oocytes injected with CHIP28 RNA exhibit high osmotic water permeability, Pf [Preston et al. (1992) Science 256,
Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
Journal:Biochemistry
PubMed ID:7849020
'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent
Luminal pH regulated calcium release kinetics in sarcoplasmic reticulum vesicles.
Journal:Biochemistry
PubMed ID:8873610
'Calcium binding to triads isolated from rabbit skeletal muscle followed a single hyperbolic function in the pH range 5.5-8.0. Maximal binding was obtained at pH 8.0; decreasing the pH decreased the binding capacity and, at pH < or = 6.0, increased Kd 2-fold. These results indicate that lowering the pH