6-ROX, SE (6-Carboxy-X-Rhodamine, Succinimidyl Ester), single isomer

Citações e referências (54)

Invitrogen™

6-ROX, SE (6-Carboxy-X-Rhodamine, Succinimidyl Ester), single isomer

Número do catálogo	Quantity
C6126	5 mg

Número do catálogo C6126

Preço (BRL)

Quantity:

The isomeric 6-ROX, SE is an amine-reactive form of carboxy-X-rhodamine and is widely used for oligonucleotide labeling and automated DNA sequencingapplications.

For Research Use Only. Not for use in diagnostic procedures.

Especificações

Chemical ReactivityAmine

Label or DyeROX

Product Type6-ROX SE

Quantity5 mg

Reactive MoietyActive Ester, Succinimidyl Ester

Shipping ConditionRoom Temperature

Label TypeClassic Dyes

Unit SizeEach

Conteúdo e armazenamento

Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

What is the excitation and emission wavelength for rhodamine?

Rhodamine is a generic term for a wide variety of cationic dyes whose fluorescence emission can range from green, orange to red. The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (“EC”; cm^-1 M^-1), for various rhodamine dyes (data derived with dye dissolved in methanol).

Dye	Excitation	Emission	EC
Rhodamine B	568	583	88,000
Rhodamine 123	507	529	101,000
Rhodamine 110	499	521	92,000
Rhodamine 6G	528	551	105,000
XRITC	572	596	92,000

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citações e referências (54)

Citações e referências

Abstract

Real-time, sequence-specific detection of nucleic acids during strand displacement amplification.

Authors:Nadeau JG,Pitner JB,Linn CP,Schram JL,Dean CH,Nycz CM

Journal:Analytical biochemistry

PubMed ID:10603241

Molecular beacons: a new approach for semiautomated mutation analysis.

Authors:Giesendorf BA, Vet JA, Tyagi S, Mensink EJ, Trijbels FJ, Blom HJ

Journal:Clin Chem

PubMed ID:9510851

Molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We designed molecular beacons to detect a point mutation in the methylenetetrahydrofolate reductase (MTHFR) gene, a mutation that has been related to an increased risk for cardiovascular disease and neural tube defects. The application of molecular beacons enables fast, semiautomated, ... More

Sites of tubulin polymerization in PC12 cells.

Authors:Keith CH, Blane K

Journal:J Neurochem

PubMed ID:2179472

'The site at which tubulin enters into polymer in the neuritic process is a very important datum in terms of our understanding of the mechanism of transport of the microtubular cytoskeleton out the axon. If the form of tubulin being transported out the axon is the microtubule, then assembly of ... More

Kinetic studies by fluorescence resonance energy transfer employing a double-labeled oligonucleotide: hybridization to the oligonucleotide complement and to single-stranded DNA.

Authors:Parkhurst KM, Parkhurst LJ

Journal:Biochemistry

PubMed ID:7819209

'A single 16-base oligodeoxyribonucleotide was labeled at the 3''-end with fluorescein and at the 5''-end with x-rhodamine (R*oligo*F); the chromophores served as a donor/acceptor pair, respectively, for Förster resonance energy transfer. We exploited the striking differences in the steady-state emission spectra of the R*oligo*F as a single strand and in ... More

Microtubules of the kinetochore fiber turn over in metaphase but not in anaphase.

Authors:Gorbsky GJ, Borisy GG

Journal:J Cell Biol

PubMed ID:2668301

'In previous work we injected mitotic cells with fluorescent tubulin and photobleached them to mark domains on the spindle microtubules. We concluded that chromosomes move poleward along kinetochore fiber microtubules that remain stationary with respect to the pole while depolymerizing at the kinetochore. In those experiments, bleached zones in anaphase ... More

View all 6-ROX, SE (6-Carboxy-X-Rhodamine, Succinimidyl Ester), single isomer citations