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Citações e referências (22)
Invitrogen™
DQ™ Green BSA - Special Packaging
DQ Green BSA is a fluorogenic substrate for proteases. A strong fluorescence quenching effect is observed when proteins are heavilyLeia mais
| Número do catálogo | Quantity |
|---|---|
| D12050 | 5 x 1 mg |
Número do catálogo D12050
Preço (BRL)
4.262,54
Each
Quantity:
5 x 1 mg
Preço (BRL)
4.262,54
Each
DQ Green BSA is a fluorogenic substrate for proteases. A strong fluorescence quenching effect is observed when proteins are heavily labeled with BODIPY dyes. Upon hydrolysis of the DQ Green BSA to single, dye-labeled peptides by proteases, this quenching is relieved, producing brightly fluorescent products.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Product LineDQ
Quantity5 x 1 mg
Shipping ConditionRoom Temperature
SubstrateProtease Substrate
Detection MethodFluorescence
FormSolid
Substrate PropertiesProtein-Based Substrate
Unit SizeEach
Conteúdo e armazenamento
Store in freezer (-5 to -30°C) and protect from light.
Citações e referências (22)
Citações e referências
Abstract
Beclin1-binding UVRAG targets the class C Vps complex to coordinate autophagosome maturation and endocytic trafficking.
Journal:Nat Cell Biol
PubMed ID:18552835
'Autophagic and endocytic pathways are tightly regulated membrane rearrangement processes that are crucial for homeostasis, development and disease. Autophagic cargo is delivered from autophagosomes to lysosomes for degradation through a complex process that topologically resembles endosomal maturation. Here, we report that a Beclin1-binding autophagic tumour suppressor, UVRAG, interacts with the
A cytosolic calcium transient is not necessary for degranulation or oxidative burst in immune complex-stimulated neutrophils.
Journal:J Leukoc Biol
PubMed ID:9307071
'Receptor-mediated activation of neutrophils (PMN) initiates possibly interdependent events, including a rapid transient increase in [Ca2+]i, implicated as a second messenger. To investigate whether this transient is required for eventual degranulation, PMN were incubated with an intracellular Ca2+ chelator (BAPTA), then exposed to chemotactic peptide [N-formyl-methionyl-leucyl-phenylalanine (fMLP)l with or without
Maturation and trafficking of monocyte-derived dendritic cells in monkeys: implications for dendritic cell-based vaccines.
Journal:J Immunol
PubMed ID:10679086
'Human dendritic cells (DC) have polarized responses to chemokines as a function of maturation state, but the effect of maturation on DC trafficking in vivo is not known. We have addressed this question in a highly relevant rhesus macaque model. We demonstrate that immature and CD40 ligand-matured monocyte-derived DC have
A novel methodology for the investigation of intracellular proteolytic processing in intact cells.
Journal:Eur J Cell Biol
PubMed ID:9548376
'Taking advantage of the unique spectral properties of the fluorescent probe FL-Bodipy, we have developed a new methodology to study processing of exogenous proteins in intact cells. FL-Bodipy was conjugated to bovine serum albumin (BSA) at a molar ratio of 29 probe molecules to 1 albumin equivalent. The resulting conjugate
Assays to assess autophagy induction and fusion of autophagic vacuoles with a degradative compartment, using monodansylcadaverine (MDC) and DQ-BSA.
Journal:Methods Enzymol
PubMed ID:19200877
In this chapter we describe the use of monodasylcadaverine (MDC) and DQ-BSA, two practical and convenient tools to study the autophagic pathway. MDC is a lysosomotropic compound useful for the identification of autophagic vesicles by fluorescence microscopy and, in addition, to assess autophagy induction via the accumulation of MDC-labeled vacuoles.