ELF™ 97 Endogenous Phosphatase Detection Kit

Citações e referências (32)

Invitrogen™

ELF™ 97 Endogenous Phosphatase Detection Kit

The ELF™ 97 Endogenous Phosphatase Kit can be used to detect phosphatase activity with the ELF™ 97 phosphatase substrate thatLeia mais

Número do catálogo	Quantity
E6601	1 kit

Número do catálogo E6601

Preço (BRL)

3.440,43

Adicionar ao carrinho

Quantity:

1 kit

Preço (BRL)

3.440,43

Adicionar ao carrinho

The ELF™ 97 Endogenous Phosphatase Kit can be used to detect phosphatase activity with the ELF™ 97 phosphatase substrate that upon hydrolysis produces a bright and photostable yellow-green fluorescent precipitate at the site of enzymatic activity. This fluorescent precipitate has several unique spectral characteristics, including an extremely large Stokes shift, 180 nm that makes it easily distinguishable from endogenous fluorescence.

For Research Use Only. Not for use in diagnostic procedures.

Especificações

Detection MethodFluorescence

Dye TypeELF 97

FormLiquid

Quantity1 kit

Shipping ConditionWet Ice

SubstrateELF™ 97 phosphate

Substrate PropertiesChemical Substrate

Substrate TypePhosphatase Substrate

Target EnzymePhosphatases

ColorYellow-Green

Emission345/530

For Use With (Application)Phosphatase Assay

For Use With (Equipment)Fluorescence Microscope

Product LineELF

Product TypePhosphatase Assay

Unit SizeEach

Conteúdo e armazenamento

Store in refrigerator 2°C to 8°C and protect from light.

Frequently asked questions (FAQs)

Can the ELF 97 reagent be applied to live cells?

ELF 97 substrate is cell impermeant. It may be used on live cells that exhibit phosphatase activity on the surface of the cells, but not intracellular phosphatase activity in live cells. The alternative product for live-cell detection of phosphatase activity is the cell-permeable Alkaline Phosphatase Live Stain (Cat. No. A14353).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can ELF 97 stained cell/tissue samples be multiplexed with antibody labeling?

No. The ELF 97 reagent does not covalently attach to any cellular components and may be washed away with any subsequent antibody labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citações e referências (32)

Citações e referências

Abstract

Characterization of a new NIH-registered variant human embryonic stem cell line, BG01V: a tool for human embryonic stem cell research.

Authors:Plaia TW,Josephson R,Liu Y,Zeng X,Ording C,Toumadje A,Brimble SN,Sherrer ES,Uhl EW,Freed WJ,Schulz TC,Maitra A,Rao MS,Auerbach JM

Journal:Stem cells (Dayton, Ohio)

PubMed ID:16293579

Primitive adult hematopoietic stem cells can function as osteoblast precursors.

Authors:Olmsted-Davis EA, Gugala Z, Camargo F, Gannon FH, Jackson K, Kienstra KA, Shine HD, Lindsey RW, Hirschi KK, Goodell MA, Brenner MK, Davis AR

Journal:Proc Natl Acad Sci U S A

PubMed ID:14673088

'Osteoblasts are continually recruited from stem cell pools to maintain bone. Although their immediate precursor is a plastic-adherent mesenchymal stem cell able to generate tissues other than bone, increasing evidence suggests the existence of a more primitive cell that can differentiate to both hematopoietic and mesenchymal cells. We show here ... More

Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.

Authors:Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM

Journal:Stem Cells

PubMed ID:17284651

'As the number of human embryonic stem cell (hESC) lines increases, so does the need for systematic evaluation of each line''s characteristics and potential. Comparisons between lines are complicated by variations in culture conditions, feeders, spontaneous differentiation, and the absence of standardized assays. These difficulties, combined with the inability of ... More

Osteoclast responses to lipopolysaccharide, parathyroid hormone and bisphosphonates in neonatal murine calvaria analyzed by laser scanning confocal microscopy.

Authors:Suzuki K, Takeyama S, Kikuchi T, Yamada S, Sodek J, Shinoda H

Journal:J Histochem Cytochem

PubMed ID:16087705

'Because the development and activity of osteoclasts in bone remodeling is critically dependent on cell-cell and cell-matrix interactions, we used laser confocal microscopy to study the response of osteoclasts to lipopolysaccharide (LPS; 10 microg/ml), parathyroid hormone (PTH; 10(-8) M), and bisphosphonates (BPs; 1-25 microM clodronate or 0.1-2.5 microM risedronate) in ... More

Fluorescence-based staining for tartrate-resistant acidic phosphatase (TRAP) in osteoclasts combined with other fluorescent dyes and protocols.

Authors:Filgueira L

Journal:J Histochem Cytochem

PubMed ID:14966208

Osteoclasts are the only bone-resorbing cells. In addition to other specific properties, osteoclasts are characterized by their expression of tartrate-resistant acidic phosphatase (TRAP), which is usually detected using a histochemical method for light microscopy. Using ELF97 phosphatase substrate, this study describes a new fluorescence-based method for TRAP detection. The fluorescence-based ... More

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