Thermo Scientific Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.
Applications
• Removal of mRNA prior to synthesis of second strand cDNA • RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis • Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT) • Site-specific cleavage of RNA • Studies of in vitro polyadenylation reaction products
Especificações
Concentration
5 U/μL
Enzyme
RNase
Compatible Buffer
10X Reaction Buffer
Quantity
500 U
Product Type
RNase H
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