Phusion Human Specimen Direct PCR Kit
Phusion Human Specimen Direct PCR Kit
Thermo Scientific™

Phusion Human Specimen Direct PCR Kit

Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.
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Número do catálogoQuantity
F150BID200 reactions
Número do catálogo F150BID
Preço (BRL)
2.950,90
Each
Adicionar ao carrinho
Quantity:
200 reactions
Request bulk or custom format
Preço (BRL)
2.950,90
Each
Adicionar ao carrinho
Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples. A tiny amount of sample is used directly in the PCR reaction with no prior purification steps. This allows significant savings in both time and cost.

Tested sample materials include buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth, and skin biopsies. The kit is compatible with fresh, frozen, and even formalin-fixed, paraffin-embedded (FFPE) human tissue samples. The Phusion Human Specimen Direct PCR Kit includes optimized reagents and detailed protocols for many sample materials. The modified Phusion Hot Start II High-Fidelity DNA Polymerase utilized in the kit is a highly accurate proofreading DNA polymerase, and PCR products obtained are perfectly suitable even for cloning purposes.

Features

  • No need for time-consuming and expensive DNA purification steps
  • Two simple protocols for various applications
  • Extremely short PCR protocol times
  • Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product

Notes

  • The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based DNA polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Hot StartBuilt-In Hot Start
No. of Reactions200 reactions
OverhangBlunt
PolymerasePhusion Human Specimen DNA Polymerase
Product TypeDirect PCR Kit
Quantity200 reactions
Reaction FormatSeparate Components
Sample TypeHuman
Shipping ConditionWet or Dry Ice
Starting MaterialDNA
For Use With (Application)Hot-start PCR
GC-Rich PCR PerformanceHigh
PCR MethodDirect PCR
Reaction SpeedFast
Unit SizeEach
Conteúdo e armazenamento
• Phusion Human Specimen DNA Polymerase, 80 μL
• 2X Phusion Human Specimen PCR Buffer, 2 x 1.25 mL
• Universal Control Primer Mix (25 μM), 40 μL
• Dilution Buffer, 2 x 5 mL
• DNARelease Additive, 3 x 100 μL

Store at -20°C.

Frequently asked questions (FAQs)

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

How are samples, stored at different conditions, compatible with the Phire Tissue Direct PCR Master Mix?

With the Phire Tissue Direct PCR Master Mix you can amplify DNA from differently stored tissues (fresh, frozen, FFPE) from human and a variety of animal species.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.

View all Phusion Human Specimen Direct PCR Kit FAQs