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Phire Tissue Direct PCR Master Mix
Thermo Scientific™

Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
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Número do catálogoNo. of Reactions
F170L1250 Reactions
F170S250 Reactions
Número do catálogo F170L
Preço (BRL)
8.517,79
Each
No. of Reactions:
1250 Reactions
Request bulk or custom format
Preço (BRL)
8.517,79
Each
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Features

  • Direct PCR reduces time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
  • Specially engineered Phire DNA polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

Notes

  • The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Hot StartBuilt-In Hot Start
Includes Label or DyeYes
No. of Reactions1250 Reactions
OverhangBlunt
PolymerasePhire Hot Start II DNA Polymerase
Product TypeDirect PCR Master Mix
Quantity1,250 reactions
Reaction FormatSuperMix or Master Mix
Sample TypeTissue
Shipping ConditionWet or Dry Ice
Starting MaterialDNA
Concentration2X
For Use With (Application)Genotyping, Hot-start PCR
GC-Rich PCR PerformanceMedium
PCR MethodDirect PCR
Reaction SpeedFast
Unit SizeEach
Conteúdo e armazenamento
• 2X Phire Tissue Direct PCR Master Mix, 10 x 1.25 mL
• Dilution Buffer, 2 x 5 mL
• DNARelease Additive, 1.3 mL
• 25 μM Control Primer Mix, 40 μL
• Nuclease-free water, 10 x 1.25 mL
• 50 μg O'GeneRuler Express DNA Ladder

Store at -20°C.

Frequently asked questions (FAQs)

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

How are samples, stored at different conditions, compatible with the Phire Tissue Direct PCR Master Mix?

With the Phire Tissue Direct PCR Master Mix you can amplify DNA from differently stored tissues (fresh, frozen, FFPE) from human and a variety of animal species.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.

View all Phire Tissue Direct PCR Master Mix FAQs