Search
Citações e referências (99)
Invitrogen™
FluoReporter™ lacZ Flow Cytometry Kit
The FluoReporter™ lacZ Flow Cytometry Kit provides materials and protocols for quantitating β-galactosidase activity with fluorescein di-V-galactoside (FDG) in singleLeia mais
| Número do catálogo | Quantity |
|---|---|
| F1930 conhecido também como F-1930 | 1 Kit |
Número do catálogo F1930
conhecido também como F-1930
Preço (BRL)
-
Quantity:
1 Kit
The FluoReporter™ lacZ Flow Cytometry Kit provides materials and protocols for quantitating β-galactosidase activity with fluorescein di-V-galactoside (FDG) in single cells using flow cytometry. This kit is accompanied by a license to practice patented techniques for loading FDG by hypotonic shock and improving retention of fluorescein in lacZ-positive cells.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Detection MethodFluorescence
FormSolution
Product LineFluoReporter
Product Typeβ-Galactosidase Reporter Gene Assay System
Quantity1 Kit
Shipping ConditionRoom Temperature
Unit SizeEach
Conteúdo e armazenamento
Contains 1 vial FDG (0.5 mL, 20 mM in water/DMSO/ethanol, 8:1:1, v/v), 1 vial PETG (2.0 mL, 50 mM in water), chloroquine diphosphates (1.0 mL, 30 mM in water) and 1 vial of propidium iodide (1.0 mL, 150 μM in water).
Store in freezer (-5 to -30°C) and protect from light.
Store in freezer (-5 to -30°C) and protect from light.
Citações e referências (99)
Citações e referências
Abstract
Detection of rare antigen-presenting cells by the lacZ T-cell activation assay suggests an expression cloning strategy for T-cell antigens.
Journal:Proc Natl Acad Sci U S A
PubMed ID:1378619
'The alpha/beta T-cell receptor a complex ligand formed by the association of antigenic peptides with molecules of the major histocompatibility complex (MHC). The inherent limitations of the conventional T-cell activation assays used to detect these peptide/MHC ligands have, until now, hampered the development of expression cloning systems for T-cell antigens.
Primitive adult hematopoietic stem cells can function as osteoblast precursors.
Journal:Proc Natl Acad Sci U S A
PubMed ID:14673088
'Osteoblasts are continually recruited from stem cell pools to maintain bone. Although their immediate precursor is a plastic-adherent mesenchymal stem cell able to generate tissues other than bone, increasing evidence suggests the existence of a more primitive cell that can differentiate to both hematopoietic and mesenchymal cells. We show here
Retroviral gene transfer is inhibited by chondroitin sulfate proteoglycans/glycosaminoglycans in malignant pleural effusions.
Journal:J Biol Chem
PubMed ID:9115227
'Gene therapy may be an important adjuvant for treating cancer in the pleural space. The initial results of retroviral gene transfer to cancer cells in malignant pleural effusions revealed that transduction was markedly inhibited, and studies to characterize the inhibitory factor(s) were performed. The inhibition was contained within the soluble,
In situ detection of transcriptionally active chromatin and genetic regulatory elements in individual viable mammalian cells.
Journal:Immunol Suppl
PubMed ID:2807403
'Using a newly developed FACS method for quantifying the expression of the Escherischia coli lacZ reporter gene in viable mammalian cells, we have obtained cloned cell lines in which the expression of lacZ is under the control of native endogenous transcription elements. We infected the murine pre-B cell 70Z/3 with
Transcriptional activity of the neuron-specific enolase (NSE) promoter in murine embryonic stem (ES) cells and preimplantation embryos.
Journal:Eur J Cell Biol
PubMed ID:7925488
'Mouse embryonic stem (ES) cells were transfected with a plasmid composed of an E. coli lacZ gene fused to 1.8 kb of rat neuron-specific enolase (NSE) promoter sequences. While this reporter construct had been shown previously to function exclusively in postmitotic neurons and neuro-endocrine cells of transgenic mice, stably transfected