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Citações e referências (7)
Invitrogen™
Fluorescein Di-β-D-Glucuronide (FDGlcU)
Cell permeant
| Número do catálogo | Quantity |
|---|---|
| F2915 conhecido também como F-2915 | 5 mg |
Número do catálogo F2915
conhecido também como F-2915
Preço (BRL)
-
Quantity:
5 mg
Fluorescein di-β-D-glucuronide (FDGlcU) is colorless and nonfluorescent until it is hydrlozed to the monoglucuronide and then to the highly fluorescent fluorescein.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Cell PermeabilityCell-permeant
Excitation/Emission490/514
Quantity5 mg
Shipping ConditionRoom Temperature
SubstrateBeta-Glucuronidase (GUS) Substrate
Detection MethodFluorescence
Emission Wavelength Range517 nm
Excitation Wavelength Range490 nm
Substrate PropertiesChemical Substrate
Unit SizeEach
Conteúdo e armazenamento
Store in freezer (-5 to -30°C).
Frequently asked questions (FAQs)
Can I make a higher stock solution of Fluorescein Di-β-D-Glucuronide (FDGlcU) (Cat. No. F2915) e.g., 50 mM in PBS?
What is the molecular weight of Fluorescein Di-β-D-Glucuronide (FDGlcU) (Cat. No. F2915)?
Citações e referências (7)
Citações e referências
Abstract
Single cell analysis and selection of living retrovirus vector-corrected mucopolysaccharidosis VII cells using a fluorescence-activated cell sorting-based assay for mammalian beta-glucuronidase enzymatic activity.
Journal:J Biol Chem
PubMed ID:9872999
'Mutations in the acid beta-glucuronidase gene lead to systemic accumulation of undegraded glycosaminoglycans in lysosomes and ultimately to clinical manifestations of mucopolysaccharidosis VII (Sly disease). Gene transfer by retrovirus vectors into murine mucopolysaccharidosis VII hematopoietic stem cells or fibroblasts ameliorates glycosaminoglycan accumulation in some affected tissues. The efficacy of gene
Gene expression imaging by enzymatic catalysis of a fluorescent probe via membrane-anchored beta-glucuronidase.
Journal:Gene Ther
PubMed ID:17235292
'Development of nonimmunogenic and specific reporter genes to monitor gene expression in vivo is important for the optimization of gene therapy protocols. We developed a membrane-anchored form of mouse beta-glucuronidase (mbetaG) as a reporter gene to hydrolyze a nonfluorescent glucuronide probe (fluorescein di-beta-D-glucuronide, (FDGlcU) to a highly fluorescent reporter to
Enzyme-generated intracellular fluorescence for single-cell reporter gene analysis utilizing Escherichia coli beta-glucuronidase.
Journal:Cytometry
PubMed ID:8866216
'We report the development of a new fluorescence-activated cell sorter (FACS)-based reporter gene system utilizing the enzymatic activity of the E. coli beta-glucuronidase (gus) gene. When loaded with the Gus substrate fluorescein-di-beta-D-glucuronide (FDGlcu), individual mammalian cells expressing and translating gus mRNA liberate sufficient levels of intracellular fluorescein for quantitative analysis
Direct influence of S9 liver homogenate on fluorescence signals: impact on practical applications in a bacterial genotoxicity assay.
Journal:Mutat Res
PubMed ID:11719102
Assays based on the bacterial SOS-response offer the possibility of automatization of genotoxicity testing for screening of large compound libraries. While existing assays use colorimetric detection or luminescence read-out, we describe here the use of a fluorescence-based system to achieve high sensitivity of detection required for assay miniaturization. Three commonly
Critical elements of oligosaccharide acceptor substrates for the Pasteurella multocida hyaluronan synthase.
Journal:J Biol Chem
PubMed ID:16361253
Three-dimensional structures are not available for polysaccharide synthases and only minimal information on the molecular basis for catalysis is known. The Pasteurella multocida hyaluronan synthase (PmHAS) catalyzes the polymerization of the alternating beta1,3-N-acetylglucosamine-beta1,4-glucuronic acid sugar chain by the sequential addition of single monosaccharides to the non-reducing terminus. Therefore, PmHAS possesses