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Citações e referências (14)
Invitrogen™
FluoReporter™ Blue Fluorometric dsDNA Quantitation Kit, 200-2,000 assays
The FluoReporter™ Blue Fluorometric dsDNA Quantitation Kit provides the protocols and reagents for analyzing cellular NA with the blue-fluorescent HoechstLeia mais
| Número do catálogo | Quantity |
|---|---|
| F2962 | 1 kit |
Número do catálogo F2962
Preço (BRL)
-
Quantity:
1 kit
The FluoReporter™ Blue Fluorometric dsDNA Quantitation Kit provides the protocols and reagents for analyzing cellular NA with the blue-fluorescent Hoechst 33258 nucleic acid stain. With this kit, quantitation of cellular DNA is rapid, and all manipulations can be carried out in microplate wells. The cells are lysed by freezing them in distilled water, the diluted dye solution is then added to the lysed cells, and fluorescence is measured.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
For Use With (Equipment)Microplate Reader
Product LineFluoReporter
Product TypedsDNA Quantitation Kit
Quantity1 kit
Shipping ConditionRoom Temperature
Detection MethodFluorescence
Format96-well Plate
Label or DyeOther Labels or Dyes
Unit SizeEach
Conteúdo e armazenamento
Store in refrigerator (2–8°C) and protect from light.
Frequently asked questions (FAQs)
Can I use Hoechst dyes to do both DNA quantitation and cell number counting in plates?
Citações e referências (14)
Citações e referências
Abstract
Dominant-negative C/EBP disrupts mitotic clonal expansion and differentiation of 3T3-L1 preadipocytes.
Journal:Proc Natl Acad Sci U S A
PubMed ID:14688407
'Hormonal induction of growth-arrested 3T3-L1 preadipocytes rapidly activates expression of CCAAT/enhancer-binding protein (C/EBP) beta. Acquisition of DNA-binding activity by C/EBPbeta, however, is delayed until the cells synchronously enter the S phase of mitotic clonal expansion (MCE). After MCE, C/EBPbeta activates expression of C/EBPalpha and peroxisome proliferator-activated receptor gamma, which then
In vivo imaging platform for tracking immunotherapeutic cells.
Journal:Nat Biotechnol
PubMed ID:16041364
Cellular therapeutics show great promise for the treatment of disease, but few noninvasive techniques exist for monitoring the cells after administration. Here we present a magnetic resonance imaging (MRI) technology that uses perfluoropolyether (PFPE) agents to track cells in vivo. Fluorine MRI selectively images only the labeled cells, and a
ATP regulates the differentiation of mammalian skeletal muscle by activation of a P2X5 receptor on satellite cells.
Journal:J Cell Biol
PubMed ID:12135987
ATP is well known for its role as an intracellular energy source. However, there is increasing awareness of its role as an extracellular messenger molecule (Burnstock, 1997). Although evidence for the presence of receptors for extracellular ATP on skeletal myoblasts was first published in 1983 (Kolb and Wakelam), their physiological
5-HT2A receptor induces ERK phosphorylation and proliferation through ADAM-17 tumor necrosis factor-alpha-converting enzyme (TACE) activation and heparin-bound epidermal growth factor-like growth factor (HB-EGF) shedding in mesangial cells.
Journal:J Biol Chem
PubMed ID:16737974
In this study, we present multiple lines of evidence to support a critical role for heparin-bound EGF (epidermal growth factor)-like growth factor (HB-EGF) and tumor necrosis factor-alpha-converting enzyme (TACE) (ADAM17) in the transactivation of EGF receptor (EGFR), ERK phosphorylation, and cellular proliferation induced by the 5-HT(2A) receptor in renal mesangial
Small proline-rich repeat 3 is a novel coordinator of PDGFRβ and integrin β1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts.
Journal:FASEB J
PubMed ID:32297675