Phusion™ HF and GC Buffer Packs
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Thermo Scientific™

Phusion™ HF and GC Buffer Packs

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
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Número do catálogoChemical Name or MaterialColor
F519LGC Buffer PackColorless
F518LHF Buffer PackColorless
F520LDetergent-free FF Buffer PackColorless
F521LDetergent-free GC Buffer PackColorless
F538LHF Buffer PackGreen
F539LGC Buffer PackGreen
Número do catálogo F519L
Preço (BRL)
285,66
Each
Adicionar ao carrinho
Chemical Name or Material:
GC Buffer Pack
Color:
Colorless
Request bulk or custom format
Preço (BRL)
285,66
Each
Adicionar ao carrinho
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

  • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
  • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Chemical Name or MaterialGC Buffer Pack
ColorColorless
DescriptionPhusion™ GC Buffer Pack
Includes• 5X Phusion GC Reaction Buffer (4 x 1.5 mL)
• 50 mM MgCl2 (4 x 1.5 mL)
• DMSO (0.5 mL)

Store at –20°C.
For Use With (Application)Standard PCR
Quantity4 x 1.5 mL
Unit SizeEach