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Citações e referências (19)
Invitrogen™
FluoSpheres™ Size Kit #1, Carboxylate-modified Microspheres, red fluorescent (580/605), 2% solids, six sizes
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed fromLeia mais
| Número do catálogo | Quantity |
|---|---|
| F8887 | 6 x 1 mL (1 mL/size) |
Número do catálogo F8887
Preço (BRL)
-
Quantity:
6 x 1 mL (1 mL/size)
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes™ FluoSpheres™ beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. This FluoSpheres™ Size Kit supplies 1 mL of each the 0.02, 0.1, 0.2, 0.5, 1.0, and 2.0 μm sizes.
FluoSpheres™ Microsphere Specifications
- Label (Ex/Em): Red fluorescent (580/605)
- Nominal bead diameter: 0.02, 0.1, 0.2, 0.5, 1.0, 2.0 μm
- Coupling surface: Carboxylate
- Solids: 2%
Characteristics of the Various FluoSpheres™ Coupling Surfaces
- Carboxylate-modified FluoSpheres™ beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.
- Sulfate FluoSpheres™ beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin.
- Aldehyde—sulfate FluoSpheres™ beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
- Amine-modified FluoSpheres™ beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide.
Key Applications of Microspheres
- Instrument calibration (flow cytometry, microscopy, HTS, HCS)
- Flow testing (microfluidics, blood flow, water flow, and air flow)
- Cell biology tracers (cell differentiation and cell tracing)
- Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)
Choices for FluoSpheres™ Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you'll find beads with these variations:
- Ten fluorescent colors
- Ten nominal bead diameters: 0.02 μm, 0.04 μm, 0.1 μm, 0.2 μm, 0.5 μm, 1.0 μm, 2.0 μm, 4.0 μm, 10.0 μm, and 15.0 μm
- Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
- Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum
Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.
We'll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres™ beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 13485:2000 certified.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Product LineFLUOSPHERES
Quantity6 x 1 mL (1 mL/size)
Shipping ConditionRoom Temperature
Surface ModificationCarboxylate
ColorRed
Diameter (Metric)0.02, 0.1, 0.2, 0.5, 1, 2 μm
MaterialPolystyrene
Product TypeCarboxylate-Modified Microsphere
Unit SizeEach
Conteúdo e armazenamento
Store in refrigerator (2°C to 8°C) and protect from light.
Frequently asked questions (FAQs)
What is the warranty for FluoSpheres microspheres?
After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?
I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?
I accidentally froze my microspheres; can I still use them?
My protein-coated microspheres appear to be non-specifically binding in my experimental system. Do you have a product that can help reduce these non-specific interactions?
Citações e referências (19)
Citações e referências
Abstract
Tuftsin binds neuropilin-1 through a sequence similar to that encoded by exon 8 of vascular endothelial growth factor.
Journal:The Journal of biological chemistry
PubMed ID:16371354
Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion.
Journal:Mol Biol Cell
PubMed ID:10982406
'Protein traffic from the cell surface or the trans-Golgi network reaches the lysosome via a series of endosomal compartments. One of the last steps in the endocytic pathway is the fusion of late endosomes with lysosomes. This process has been reconstituted in vitro and has been shown to require NSF,
Fractal nature of regional ventilation distribution.
Journal:J Appl Physiol
PubMed ID:10797111
'High-resolution measurements of pulmonary perfusion reveal substantial spatial heterogeneity that is fractally distributed. This observation led to the hypothesis that the vascular tree is the principal determinant of regional blood flow. Recent studies using aerosol deposition show similar ventilation heterogeneity that is closely correlated with perfusion. We hypothesize that ventilation
Mapping mechanical strain of an endogenous cytoskeletal network in living endothelial cells.
Journal:Biophys J
PubMed ID:12668477
'A central aspect of cellular mechanochemical signaling is a change of cytoskeletal tension upon the imposition of exogenous forces. Here we report measurements of the spatiotemporal distribution of mechanical strain in the intermediate filament cytoskeleton of endothelial cells computed from the relative displacement of endogenous green fluorescent protein (GFP)-vimentin before
Viewing dynamic assembly of molecular complexes by multi-wavelength single-molecule fluorescence.
Journal:Biophys J
PubMed ID:16698779
'Complexes of macromolecules that transiently self-assemble, perform a particular function, and then dissociate are a recurring theme in biology. Such systems often have a large number of possible assembly/disassembly intermediates and complex, highly branched reaction pathways. Measuring the single-step kinetic parameters in these reactions would help to identify the functionally