Search
Citações e referências (14)
Invitrogen™
HCS Mitochondrial Health Kit
The HCS Mitochondrial Health Kit simultaneously and quantitatively measures two important cell-health parameters: mitotoxicity and cytotoxicity. The MitoHealth stain detectsLeia mais
| Número do catálogo | Quantity |
|---|---|
| H10295 | 1 kit |
Número do catálogo H10295
Preço (BRL)
4.202,29
Each
Quantity:
1 kit
Preço (BRL)
4.202,29
Each
The HCS Mitochondrial Health Kit simultaneously and quantitatively measures two important cell-health parameters: mitotoxicity and cytotoxicity. The MitoHealth stain detects changes in the mitochondrial membrane potential, whereupon the reagent accumulates in active mitochondria. The kit includes the Image-iT® DEAD™ Green to detect cytotoxicity, an impermeant dye to healthy cells that becomes permeant when the plasma membrane of cells is compromised. Both indicators are amenable to fixation and permeabilization, thus allowing for multiplexing with other biomarkers of interest. The blue-fluorescent nuclear segmentation tool Hoechst 33342 is also included and stains DNA in both live and dead cells.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Detection MethodFluorescence
Dye TypeImage-iT™ DEAD Green viability stain, MitoHealth stain, Hoechst 33342
Format96-well plate
Quantity1 kit
Shipping ConditionRoom Temperature
ColorOrange, Blue, Green
For Use With (Equipment)Fluorescence Microscope, High Content Analysis Instrument
Product LineMolecular Probes
Product TypeMitochondrial Health Kit
Unit SizeEach
Conteúdo e armazenamento
The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.Store at ≤-20°C, desiccate and protect from light.
Frequently asked questions (FAQs)
What is the fluorescence excitation/emission maxima of the stains in the HCS mitochondrial health kit?
Citações e referências (14)
Citações e referências
Abstract
Nullomer derived anticancer peptides (NulloPs): differential lethal effects on normal and cancer cells in vitro.
Journal:Peptides
PubMed ID:23000474
'We demonstrate the first use of the nullomer (absent sequences) approach to drug discovery and development. Nullomers are the shortest absent sequences determined in a species, or group of species. By identifying the shortest absent peptide sequences from the NCBI databases, we screened several potential anti-cancer peptides. In order to
High Content Analysis technology for evaluating the joint toxicity of sunset yellow and sodium sulfite in vitro.
Journal:Food Chem
PubMed ID:28530558
'Foods contain various additives that affect our daily lives. At present, food additive safety evaluation standards are based on the toxicity of single additives, but food additives are often used in combination and may have additive, synergistic or antagonistic actions. The current study investigated the toxicity of food additives and
Vitamin D regulates prostate cell metabolism via genomic and non-genomic mitochondrial redox-dependent mechanisms.
Journal:J Steroid Biochem Mol Biol
PubMed ID:31574299
'Vitamin D deficiency has been associated with increased risk for aggressive prostate cancer (PCa). Prostate epithelium has a unique metabolism compared to other tissues. Normal prostate exhibits low levels of mitochondrial respiration and there is a metabolic switch to increased oxidative phosphorylation in PCa. 25-hydroxyvitamin D (25(OH)D) is the major
Handling and Assessment of Human Primary Prostate Organoid Culture.
Journal:J Vis Exp
PubMed ID:30735176
'This paper describes a detailed protocol for three-dimensional (3D) culturing, handling, and evaluation of human primary prostate organoids. The process involves seeding of epithelial cells sparsely in a 3D matrix gel on a 96-well microplate with media changes to cultivate expansion into organoids. Morphology is then assessed by whole-well capturing
A high throughput imaging database of toxicological effects of nanomaterials tested on HepaRG cells.
Journal:Sci Data
PubMed ID:31048742
'The large amount of existing nanomaterials demands rapid and reliable methods for testing their potential toxicological effect on human health, preferably by means of relevant in vitro techniques in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows deriving much more information