Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
• Convenient, ready-to-use mix • Thermostable—half life is more than 40 min at 95°C • Generates PCR products with 3'-dA overhangs • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Applications
• Routine PCR amplification of DNA fragments up to 5 kb • High throughput PCR • DNA labeling
Note
• The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
Especificações
GC-Rich PCR Performance
Low
Polymerase
Taq DNA Polymerase
Reaction Speed
Standard
Product Type
PCR Master Mix (2X)
Quantity
1000 Reactions
Fidelity (vs. Taq)
1 X
Hot Start
No
Overhang
3'-A
Reaction Format
SuperMix or Master Mix
Conteúdo e armazenamento
Contains: • 20 x 1.25 mL PCR Master Mix (2X), which includes: — Taq DNA polymerase (0.05 U/μL), — reaction buffer, — 4 mM MgCl2, and — 0.4 mM of each dNTP • 20 x 1.25 mL Nuclease-free water
Store at -20°C.
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