Thermo Scientific DreamTaq Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl₂, and dNTPs. The master mix retains all features of DreamTaq DNA Polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.

Highlights

• Direct loading of PCR product on gel
• High yields and high sensitivity of PCR
• Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
• Robust amplification with minimal optimization
• Incorporates modified nucleotides, but does not incorporate dUTP

Applications

• Routine PCR amplification of DNA fragments up to 6 kb
• Genotyping
• High throughput PCR