The CellSensor® ARE-bla Hep G2 cell line is responsive to tBHQ and Sulforaphane and can be used for analyzing the Nrf2/antioxidant response signaling pathway using the GeneBLAzer® Loading Substrates. Reactive oxygen species (ROS) can damage biological macromolecules and are detrimental to cellular health. Electrophilic compounds, xenobiotics and antioxidants are sources of reactive oxygen species, creating oxidative stress that can harm cells. Enzymes are involved in the Phase II detoxification of xenobiotics to reduce cellular stress include glutathione transferases, quinone reductase, epoxide hydrolase, heme oxygenase, UDP-glucuronosyl transferases, and gamma-glutamylcysteine synthetase. Expression of these genes protects cells from oxidative damage and can prevent mutagenesis and cancer. Transcription of these enzymes is coordinately regulated through antioxidant response elements (AREs). Nrf2 (NF-E2-related factor 2) and Nrf1 are transcription factors that bind to AREs and activate these genes. The CellSensor™ ARE-bla Hep G2 cell line contains a beta-lactamase reporter gene under control of the Antioxidant Response Element (ARE) stably integrated into Hep G2 cells. To construct this cell line, the ARE-bla construct was transduced into Hep G2 cells. Flow cytometry was used to isolate cells responsive to tert-butylhydroquinone (tBHQ). This cell line is tested under various experimental conditions, including DMSO concentration, cell number, stimulation time, substrate loading time, and functionally validated for Z'; and EC50 concentrations of tBHQ. Academic and non-profit customers, please inquire for special pricing.