PureLink™ PCR Micro Kit
PureLink™ PCR Micro Kit
Invitrogen™

PureLink™ PCR Micro Kit

PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions. These highlyLeia mais
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Número do catálogoQuantity
K31005050 Preps
K310250250 Preps
Número do catálogo K310050
Preço (BRL)
1.224,27
Each
Adicionar ao carrinho
Quantity:
50 Preps
Preço (BRL)
1.224,27
Each
Adicionar ao carrinho

PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions. These highly purified DNA fragments and amplicons provide excellent results in downstream applications such as DNA sequencing, restriction digestion, cloning, PCR, and labeling. PureLink PCR Micro Kits provide a simple and rapid method for purifying and concentrating DNA fragments ranging in size from 100 bp-12 kb.

The unique micro-nozzle design allows up to 95% recovery of DNA fragments with as little as a 5 μL elution volume. With less than a 10% coefficient of variance in the elution volume you can be sure that no ethanol is carried over from the wash steps giving you the best chance of success in downstream applications. Removal of primers, dNTPs, enzymes, and salts exceeds 98.5%. This kit can also efficiently remove unincorporated dye-labeled nucleotides from a cDNA labeling reaction.

For Research Use Only. Not for use in diagnostic procedures.
Especificações
Elution Volume Max20 μL
Elution Volume Min5 μL
FormatSpin Column
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Isolation TechnologySilica Spin Column
No. of Reactions50 Preps
Product LinePureLink
Product TypePCR Micro Kit
Quantity50 Preps
Run Time10 min.
Sample TypecDNA Labeling Reactions
Separation Range100 bp to 12 kb
Shipping ConditionRoom Temperature
Starting Material Volume125 μL
TargetDNA from Enzymatic Reactions (e.g. PCR)
For Use With (Application)Cloning, Standard PCR
Unit SizeEach
Conteúdo e armazenamento
Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.

Frequently asked questions (FAQs)

What are the size limitations for the PureLink PCR Cleanup Micro Kit?

The size cut-off is 100 bp to 12 kb.

What are the minimum and maximum size ranges for DNA fragment cleanup using the PureLink PCR Clean-Up Kit?

The size range is between 100 bp and 12 kb. Our kit comes with two buffers, Binding Buffer (B2) and Binding Buffer HC (B3). The Binding Buffer HC (B3) eliminates primer-dimers and short failed PCR products that are smaller than 300 bp.