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Citações e referências (415)
Invitrogen™
NBD-PE (N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt)
The phospholipid, NBD-PE is labeled on the head group with the environment sensitive fluorophore, NBD (excitation/emission maxima ∼463/536 nm).Prepare stockLeia mais
| Número do catálogo | Quantity |
|---|---|
| N360 | 10 mg |
Número do catálogo N360
Preço (BRL)
1.740,26
Each
Quantity:
10 mg
Preço (BRL)
1.740,26
Each
The phospholipid, NBD-PE is labeled on the head group with the environment sensitive fluorophore, NBD (excitation/emission maxima ∼463/536 nm).
Prepare stock solutions of phosphoethanolamines in ethanol to attain a stock concentration of 1 to 2 mg/mL. Sonication may be necessary to aid dispersion.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Chemical Name or MaterialPhospholipids
Excitation/Emission463/536 nm
Recommended StorageStore in freezer (-5°C to -30°C) and protect from light.
Molecular Weight (g/mol)956.25
Physical FormSolid
Product LineInvitrogen
Quantity10 mg
Unit SizeEach
Citações e referências (415)
Citações e referências
Abstract
Journal:
PubMed ID:18198177
Poly(aspartic acid)-dependent fusion of liposomes bearing the quaternary ammonium detergent [[[(1,1,3,3-tetramethylbutyl)cresoxy]ethoxy]ethyl] dimethylbenzylammonium hydroxide.
Journal:Biochemistry
PubMed ID:3349055
Addition of the quaternary ammonium detergent [[[(1,1,3,3-tetramethylbutyl)cresoxy]ethoxy]ethyl] dimethylbenzylammonium hydroxide (DEBDA[OH]) and the fluorescent probes N-(7-nitro-2-1,3-benzoxadiazol-4-yl)phosphatidylethanolamine and N-(lissamine rhodamine B sulfonyl)phosphatidylethanolamine (N-NBD-PE and N-Rh-PE, respectively) to liposomes composed of phosphatidylcholine (PC) and cholesterol (chol) resulted in the formation of fluorescently labeled liposomes bearing DEBDA[OH]. Incubation of the anionic polymer poly(aspartic acid)
Membrane vesicles containing the Sendai virus binding glycoprotein, but not the viral fusion protein, fuse with phosphatidylserine liposomes at low pH.
Journal:Biochemistry
PubMed ID:3021204
Membrane vesicles containing the Sendai virus hemagglutinin/neuraminidase (HN) glycoprotein were able to induce carboxyfluorescein (CF) release from loaded phosphatidylserine (PS) but not loaded phosphatidylcholine (PC) liposomes. Similarly, fluorescence dequenching was observed only when HN vesicles, bearing self-quenched N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine (N-NBD-PE), were incubated with PS but not PC liposomes. Thus, fusion between
Order in supported phospholipid monolayers detected by the dichroism of fluorescence excited with polarized evanescent illumination.
Journal:Biophys J
PubMed ID:6518254
A technique is described and demonstrated for measuring the orientation distribution of fluorescent molecules in a two-dimensional system. A laser beam is totally internally reflected at the interface between a glass slide and an aqueous solution, which creates a thin layer of evanescent illumination that excites fluorescent molecules near the
Lateral mobility of phospholipids in the external and internal leaflets of normal and hereditary spherocytic human erythrocytes.
Journal:Biochim Biophys Acta
PubMed ID:6466671
The lateral diffusion coefficients (D) and the mobile fractions of the fluorescent phospholipid N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)phosphatidylethanolamine (NBD-PE) and of membrane proteins labelled with fluorescein isothiocyanate, were measured by fluorescence photobleaching recovery on erythrocytes from healthy persons and from a hereditary spherocytosis patient. Measurements of lipid probe mobility were performed on ghosts labelled