Applied Biosystems™

AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2

Número do catálogo: N8080241
Applied Biosystems™

AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2

Número do catálogo: N8080241
Número do catálogo
N8080241
Tamanho da unidade
250 units
Preço (BRL)
Preço 1.801,20
Seu Preço
Disponibilidade
***
Quantidade
Número do catálogo
Tamanho da unidade
5,000 units
Preço (BRL)
Preço 25.336,50
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Disponibilidade
***
Quantidade
Número do catálogo
Tamanho da unidade
25 x 1,000 units
Disponibilidade
***
Quantidade
-
Número do catálogo
Tamanho da unidade
1,000 units
Preço (BRL)
Preço 6.783,00
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Disponibilidade
***
Quantidade
Número do catálogo
Tamanho da unidade
3,000 units
Preço (BRL)
Preço 16.758,00
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Disponibilidade
***
Quantidade
Número do catálogoTamanho da unidadePreço (BRL)DisponibilidadeQuantidade
N8080241250 units
Preço 1.801,20
Seu Preço
***
N80802495,000 units
Preço 25.336,50
Seu Preço
***
N808025925 x 1,000 unitsEntre em contato ›***-
N80802471,000 units
Preço 6.783,00
Seu Preço
***
N80802453,000 units
Preço 16.758,00
Seu Preço
***
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AmpliTaq Gold™ DNA Polymerase is a chemically modified form of AmpliTaq™ DNA Polymerase requiring thermal activation. Features of this enzyme:

• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp™ 10X PCR Buffer II with MgCl2

Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Higher specificity, higher yield
AmpliTaq Gold™ DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold™ DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

AmpliTaq Gold™ 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold™ 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold™ DNA Polymerase, AmpliTaq Gold™ 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.

Especificações

Detection Method
Primer-probe
Format
Tube
GC-Rich PCR Performance
Low
Polymerase
AmpliTaq Gold DNA Polymerase
Reaction Speed
Standard
Exonuclease Activity
5' - 3'
Product Line
AmpliTaq Gold™
Product Type
DNA Polymerase
Quantity
250 Units
Shipping Condition
Dry Ice
Concentration
5 U/μL
Fidelity (vs. Taq)
1 X
Hot Start
Built-In Hot Start
No. of Reactions
200
Overhang
3'-A
Reaction Format
Separate Components
Size (Final Product)
5 kb or less
Starting Material
DNA

Conteúdo e armazenamento

• 1 x 250 U AmpliTaq Gold DNA Polymerase
• 1 x 1.5 mL 10X PCR Buffer II
• 1 x 1.5 mL 25 mM MgCl2

Store at -15 to -30°C.

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