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Citações e referências (92)
Invitrogen™
Oregon Green™ 488 BAPTA-1, Hexapotassium Salt, cell impermeant
A visible light-excitable calcium indicator
| Número do catálogo | Quantity |
|---|---|
| O6806 | 500 μg |
Número do catálogo O6806
Preço (BRL)
3.312,94
Each
Quantity:
500 μg
Preço (BRL)
3.312,94
Each
Oregon Green 488 BAPTA-1, hexapotassium salt is a visible light—excitable calcium indicator. This water-soluble, salt form is useful for intracellular loading by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402). The cell-permeant AM ester form of the indicator is also available (O-6807).
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Detection MethodFluorescence
Dye TypeOregon Green
Emission Wavelength523 nm
Excitation Wavelength494 nm
Quantity500 μg
Shipping ConditionRoom Temperature
For Use With (Application)Calcium Indicator
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Product LineOregon Green
Product TypeCalcium Indicator
Unit SizeEach
Conteúdo e armazenamento
Store in freezer -5°C to -30°C and protect from light.
Citações e referências (92)
Citações e referências
Abstract
Intracellular astrocyte calcium waves in situ increase the frequency of spontaneous AMPA receptor currents in CA1 pyramidal neurons.
Journal:J Neurosci
PubMed ID:14736858
'Spontaneous neurotransmitter release and activation of group I metabotropic glutamate receptors (mGluRs) each play a role in the plasticity of neuronal synapses. Astrocytes may contribute to short- and long-term synaptic changes by signaling to neurons via these processes. Spontaneous whole-cell AMPA receptor (AMPAR) currents were recorded in CA1 pyramidal cells
Formation and function of synapses with respect to Schwann cells at the end of motor nerve terminal branches on mature amphibian (Bufo marinus) muscle.
Journal:J Neurosci
PubMed ID:11264312
'A study has been made of the formation and regression of synapses with respect to Schwann cells at the ends of motor nerve terminal branches in mature toad (Bufo marinus) muscle. Synapse formation and regression, as inferred from the appearance and loss of N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl) pyridinium dibromide (FM1-43)-stained vesicle clusters, occurred
Ca2+ fluorescence imaging with pico- and femtosecond two-photon excitation: signal and photodamage.
Journal:Biophys J
PubMed ID:10512842
'The signal and limitations of calcium florescence imaging using nonresonant multiphoton absorption of near-infrared femto- and picosecond laser pulses were examined. The fluorescence changes of various Ca(2+)-indicators induced by transient increases of the intradendritic calcium concentration were evaluated by evoking physiological activity in neocortical neurons in rat brain slices. Photodamage
Action potentials reliably invade axonal arbors of rat neocortical neurons.
Journal:Proc Natl Acad Sci U S A
PubMed ID:10931955
'Neocortical pyramidal neurons have extensive axonal arborizations that make thousands of synapses. Action potentials can invade these arbors and cause calcium influx that is required for neurotransmitter release and excitation of postsynaptic targets. Thus, the regulation of action potential invasion in axonal branches might shape the spread of excitation in
Buffer kinetics shape the spatiotemporal patterns of IP3-evoked Ca2+ signals.
Journal:J Physiol
PubMed ID:14555715
'Ca2+ liberation through inositol 1,4,5-trisphosphate receptors (IP3Rs) plays a universal role in cell regulation, and specificity of cell signalling is achieved through the spatiotemporal patterning of Ca2+ signals. IP3Rs display Ca2+-induced Ca2+ release (CICR), but are grouped in clusters so that regenerative Ca2+ signals may remain localized to individual clusters,