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Citações e referências (15)
Invitrogen™
ProLong™ Live Antifade Reagent, for live cell imaging
Prolong Live Antifade Reagent helps prevent the loss of fluorescent signal (photobleaching) during live cell imaging.
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| Número do catálogo | Quantity |
|---|---|
| P36974 | 5 x 1 mL |
| P36975 | 1 x 1 mL |
Número do catálogo P36974
Preço (BRL)
2.181,17
Each
Quantity:
5 x 1 mL
Preço (BRL)
2.181,17
Each
ProLong Live Antifade Reagent helps prevent the loss of fluorescent signal due to photobleaching during live cell imaging through use of enzymes from the plasma membrane of E. coli. These enzymes metabolize the elements that can cause photobleaching. When dyes are illuminated, they can degrade which reduces the sample’s fluorescent intensity and leads to the creation of free radical singlet oxygen which degrades neighboring dye molecules. ProLong Live Antifade Reagent metabolizes this element, increasing the signal intensity and duration while keeping the background signal low. ProLong Live reagent has little-to-no effect on cellular viability, proliferation, or other cellular functions in experiments up to 48 hours. ProLong Live reagent provides photobleach protection in live cell experiments for fluorophores such as GFP, RFP, Hoechst 33342, MitoTracker™, LysoTracker™, and CellTracker™dyes. ProLong Live Antifade Reagent can be added to any cell culture media or buffer that is suitable for fluorescent imaging.
Especificações
Quantity5 x 1 mL
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Product LineProLong
Product TypeLive Antifade Reagent
Reagent TypeCell Imaging Reagent
Volume (Metric)1 mL
Unit SizeEach
Conteúdo e armazenamento
Provided in 5 vials, with 1 mL each. Enough for 5000 assays (0.1 mL imaging solution/assay), or 1000 assays (0.5 mL imaging solution/assay). When stored at ≤-20°C product is stable for at least 6 months, with up to 4 freeze-thaw cycles. When stored at 2–8°C product should be used within 30 days (a precipitant may form at this temperature).
Citações e referências (15)
Citações e referências
Abstract
Persistent Replication of a Chikungunya Virus Replicon in Human Cells Is Associated with Presence of Stable Cytoplasmic Granules Containing Nonstructural Protein 3.
Journal:J Virol
PubMed ID:29875241
'Chikungunya virus (CHIKV), a mosquito-borne human pathogen, causes a disabling disease characterized by severe joint pain that can persist for weeks, months, or even years in patients. The nonstructural protein 3 (nsP3) plays essential roles during acute infection, but little is known about the function of nsP3 during chronic disease.
Diatom bloom-derived biotoxins cause aberrant development and gene expression in the appendicularian chordate Oikopleura dioica.
Journal:Commun Biol
PubMed ID:30272001
'Investigating environmental hazards than could affect appendicularians is of prime ecological interest because they are among the most abundant components of the mesozooplankton. This work shows that embryo development of the appendicularian Oikopleura dioica is compromised by diatom bloom-derived biotoxins, even at concentrations in the same range as those measured
Complex bile duct network formation within liver decellularized extracellular matrix hydrogels.
Journal:Sci Rep
PubMed ID:30111800
'The biliary tree is an essential component of transplantable human liver tissue. Despite recent advances in liver tissue engineering, attempts at re-creating the intrahepatic biliary tree have not progressed significantly. The finer branches of the biliary tree are structurally and functionally complex and heterogeneous and require harnessing innate developmental processes
Exopolysaccharide production in Caulobacter crescentus: A resource allocation trade-off between protection and proliferation.
Journal:PLoS One
PubMed ID:29293585
'Complex and interacting selective pressures can produce bacterial communities with a range of phenotypes. One measure of bacterial success is the ability of cells or populations to proliferate while avoiding lytic phage infection. Resistance against bacteriophage infection can occur in the form of a metabolically expensive exopolysaccharide capsule. Here, we
Mmf1p Couples Amino Acid Metabolism to Mitochondrial DNA Maintenance in Saccharomyces cerevisiae.
Journal:MBio
PubMed ID:29487232
'A variety of metabolic deficiencies and human diseases arise from the disruption of mitochondrial enzymes and/or loss of mitochondrial DNA. Mounting evidence shows that eukaryotes have conserved enzymes that prevent the accumulation of reactive metabolites that cause stress inside the mitochondrion. 2-Aminoacrylate is a reactive enamine generated by pyridoxal 5''-phosphate-dependent