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Citações e referências (344)
Invitrogen™
Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents
Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent.
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| Número do catálogo | Quantity | Product Type | Packaging Type |
|---|---|---|---|
| P7589 | 1 mL kit | dsDNA Assay Kit | 1 x 1 mL Bottle |
| P11496 | 10 x 100 μL kit | dsDNA Assay Kit | 10 x 100 μL Tubes |
| P7581 | 1 mL | dsDNA Reagent | 1 x 1 mL Bottle |
| P11495 | 10 x 100 μL | dsDNA Reagent | 10 x 100 μL Tubes |
Número do catálogo P7589
Preço (BRL)
5.132,69
Each
Quantity:
1 mL kit
Product Type:
dsDNA Assay Kit
Packaging Type:
1 x 1 mL Bottle
Preço (BRL)
5.132,69
Each
Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers.
Rapidly detect as little as 0.25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products.
PicoGreen dsDNA quantitation reagents are
• Significantly more sensitive than UV absorbance readings, saving precious sample amounts
• Specific for dsDNA in the presence of RNA
• Easy to use—just add the dye working solution to the sample, incubate five minutes, and read
• Suitable for use in 96- and 384-well plate formats
• Compatible with most fluorescence-based microplate readers and fluorometers
• Picogreen aproximate fluorescence absorption/emission maxima is 502/523 nm, bound to dsDNA
Applications
The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation.
PicoGreen dsDNA quantitation reagents are
• Significantly more sensitive than UV absorbance readings, saving precious sample amounts
• Specific for dsDNA in the presence of RNA
• Easy to use—just add the dye working solution to the sample, incubate five minutes, and read
• Suitable for use in 96- and 384-well plate formats
• Compatible with most fluorescence-based microplate readers and fluorometers
• Picogreen aproximate fluorescence absorption/emission maxima is 502/523 nm, bound to dsDNA
Applications
The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Excitation/Emission500/525
For Use With (Equipment)Microplate Reader
No. of Reactions200 Assays (2 mL assay volume)
Packaging Type1 x 1 mL Bottle
Product LinePICOGREEN, Quant-iT
Product TypedsDNA Assay Kit
Quantitation Range50 pg to 2 μg
Quantity1 mL kit
Shipping ConditionRoom Temperature
Detection MethodFluorescence
Unit SizeEach
Frequently asked questions (FAQs)
Why am I getting negative fluorescence values with my Qubit Assays?
I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?
What is the useful pH range for Quant-iT DNA kits?
I'm trying to quantify some DNA labeled with a fluorophore. Will this work?
Does DNA length have an effect on the dsDNA assays?
Citações e referências (344)
Citações e referências
Abstract
Journal:
PubMed ID:16517648
Rapid quantification of DNA samples extracted from buccal scrapes prior to DNA profiling.
Journal:Biotechniques
PubMed ID:9232218
Antibodies highly effective in SCID mice during infection by the intracellular bacterium Ehrlichia chaffeensis are of picomolar affinity and exhibit preferential epitope and isotype utilization.
Journal:J Immunol
PubMed ID:12133967
Although often considered to be ineffective against intracellular bacteria, Abs, in the absence of lymphocytes, have been shown previously to protect SCID mice from lethal infection by the obligate intracellular bacterium Ehrlichia chaffeensis, even when administered well after infection has been established. To identify characteristics of Abs that are critical
Genome-wide loss of heterozygosity analysis from laser capture microdissected prostate cancer using single nucleotide polymorphic allele (SNP) arrays and a novel bioinformatics platform dChipSNP.
Journal:Cancer Res
PubMed ID:12941794
Oligonucleotide arrays that detect single nucleotide polymorphisms were used to generate genome-wide loss of heterozygosity (LOH) maps from laser capture microdissected paraffin-embedded samples using as little as 5 ng of DNA. The allele detection rate from such samples was comparable with that obtained with standard amounts of DNA prepared from
Molecular genetic evidence for monoclonal origin of bilateral ovarian serous borderline tumors.
Journal:Am J Pathol
PubMed ID:12651602
Patients with serous borderline tumors of the ovary often present with multiple tumors at different sites in the abdominal cavity. Whether different foci of ovarian serous borderline tumors are monoclonal in origin, arising as a consequence of spread from a single ovarian site, or whether such deposits are polyclonal and