The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Simply dilute the reagent 1:200, load 200 μL into the wells of a microplate, add 1–20 μL sample, mix, then read the fluorescence.

The assays are highly selective for double-stranded DNA over RNA, and, in the range of 0.2–100 ng for the HS assay or 4–1000 ng for the BR assay, the fluorescence signal is linear with DNA. The assays are performed at room temperature, and the signal is stable for three hours. Common contaminants such as salts, solvents, detergents, and protein are well tolerated in these assays.