Quant-iT™ dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)
Quant-iT™ dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)
Citações e referências (16)
Invitrogen™

Quant-iT™ dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)

For highly selective quantitation of double-stranded DNA over RNA, the Quant-iT dsDNA Assay Kits, high sensitivity and broad range, produce fluorescence signals that are linear in the ranges of 0.2-100 ng and 2-1000 ng of DNA, respectively.
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Número do catálogoAssayQuantitation Range
Q33130dsDNA Quantitation, BR4 to 1000 ng
Q33120dsDNA Quantitation, HS0.2 to 100 ng
Número do catálogo Q33130
Preço (BRL)
4.288,15
Each
Adicionar ao carrinho
Assay:
dsDNA Quantitation, BR
Quantitation Range:
4 to 1000 ng
Preço (BRL)
4.288,15
Each
Adicionar ao carrinho
Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and the Quant-iT Broad-Range dsDNA Assay Kit provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. These DNA assay kits are highly selective for double-stranded DNA over RNA, and, in the ranges of 0.2–100 ng DNA (for the HS dsDNA Assay Kit) and 4–1000 ng DNA (for the BR dsDNA Assay Kit), the fluorescence signal is linear.

The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Simply dilute the reagent 1:200, load 200 μL into the wells of a microplate, add 1–20 μL sample, mix, then read the fluorescence.

The assays are highly selective for double-stranded DNA over RNA, and, in the range of 0.2–100 ng for the HS assay or 4–1000 ng for the BR assay, the fluorescence signal is linear with DNA. The assays are performed at room temperature, and the signal is stable for three hours. Common contaminants such as salts, solvents, detergents, and protein are well tolerated in these assays.

For Research Use Only. Not for use in diagnostic procedures.
Especificações
AssaydsDNA Quantitation, BR
For Use With (Equipment)Microplate Reader
No. of Reactions1000 Assays (200 μL assay volume)
Product LineQuant-iT
Quantitation Range4 to 1000 ng
Quantity1 kit
Shipping ConditionRoom Temperature
Detection MethodFluorescence
Unit SizeEach

Frequently asked questions (FAQs)

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

View all Quant-iT™ dsDNA Assay Kits, high sensitivity (HS) and broad range (BR) FAQs

Citações e referências (16)

Citações e referências
Abstract
3D chromatin conformation correlates with replication timing and is conserved in resting cells.
Authors:Moindrot B, Audit B, Klous P, Baker A, Thermes C, de Laat W, Bouvet P, Mongelard F, Arneodo A,
Journal:Nucleic Acids Res
PubMed ID:22879376
'Although chromatin folding is known to be of functional importance to control the gene expression program, less is known regarding its interplay with DNA replication. Here, using Circular Chromatin Conformation Capture combined with high-throughput sequencing, we identified megabase-sized self-interacting domains in the nucleus of a human lymphoblastoid cell line, as ... More
A significant effect of the TSPY1 copy number on spermatogenesis efficiency and the phenotypic expression of the gr/gr deletion.
Authors:Shen Y, Yan Y, Liu Y, Zhang S, Yang D, Zhang P, Li L, Wang Y, Ma Y, Tao D, Yang Y,
Journal:Hum Mol Genet
PubMed ID:23307928
'AZFc deletions cause a significant phenotypic heterogeneity with respect to spermatogenesis; however, the reason for this is poorly understood. Recently, testis-specific protein Y-encoded 1 (TSPY1) copy number variation (CNV) was determined to be a potential genetic modifier of spermatogenesis. We performed a large-scale cohort study to investigate the effect of ... More
Gene expression profiling in insulinomas of Men1 beta-cell mutant mice reveals early genetic and epigenetic events involved in pancreatic beta-cell tumorigenesis.
Authors:Fontanière S, Tost J, Wierinckx A, Lachuer J, Lu J, Hussein N, Busato F, Gut I, Wang ZQ, Zhang CX,
Journal:Endocr Relat Cancer
PubMed ID:17158767
'Mutations of the MEN1 gene lead to the occurrence of multiple endocrine neoplasia type 1 (MEN1). To gain insights into the mechanisms of the tumorigenesis related to MEN1 inactivation, we have used mice in which the Men1 gene was specifically disrupted in pancreatic beta-cells. In these mice, we observed full ... More
Effects of essential oils on methane production and fermentation by, and abundance and diversity of, rumen microbial populations.
Authors:Patra AK, Yu Z,
Journal:Appl Environ Microbiol
PubMed ID:22492451
'Five essential oils (EOs), namely, clove oil (CLO), eucalyptus oil (EUO), garlic oil (GAO), origanum oil (ORO), and peppermint oil (PEO), were tested in vitro at 3 different doses (0.25, 0.50, and 1.0 g/liter) for their effect on methane production, fermentation, and select groups of ruminal microbes, including total bacteria, ... More
Normal variants of Microcephalin and ASPM do not account for brain size variability.
Authors:Woods RP, Freimer NB, De Young JA, Fears SC, Sicotte NL, Service SK, Valentino DJ, Toga AW, Mazziotta JC,
Journal:Hum Mol Genet
PubMed ID:16687438
'Normal human brain volume is heritable. The genes responsible for variation in brain volume are not known. Microcephalin (MCPH1) and ASPM (abnormal spindle-like microcephaly associated) have been proposed as candidate genes as mutations in both genes are associated with microcephaly, and common variants of each gene are apparently under strong ... More
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