EZQ™ Protein Quantitation Kit

Citações e referências (7)

Invitrogen™

EZQ™ Protein Quantitation Kit

The EZQ™ Protein Quantitation Kit provides a fluorescence-based protein assay that facilitates fast quantitation of protein samples prepared for gelLeia mais

Número do catálogo	Quantity
R33200	2000 Assays

Número do catálogo R33200

Preço (BRL)

4.683,47

Adicionar ao carrinho

Quantity:

2000 Assays

Request bulk or custom format

Preço (BRL)

4.683,47

Adicionar ao carrinho

The EZQ™ Protein Quantitation Kit provides a fluorescence-based protein assay that facilitates fast quantitation of protein samples prepared for gel electrophoresis. The assay can be performed in the presence of detergents, urea and reducing agents—simply spot 1 μL of your protein sample onto the prepared paper, stain with our proprietary fluorescent dye, and measure the fluorescence. Samples can be quantitated by comparison with a standard curve. For added versatility, we provide a specially-designed 96-well microplate for easy quantitation of samples on a microplate reader or a laser scanner.

For Research Use Only. Not for use in diagnostic procedures.

Especificações

AssayFluorescent Protein Assay

For Use With (Application)Protein Quantitation Assay

For Use With (Equipment)Microplate Reader

Product LineEZQ

Product TypeProtein Quantitation Assay

Quantity2000 Assays

Shipping ConditionRoom Temperature

Storage RequirementsStore at room temperature. Protect from light.

Sufficient For2000 Assays

Detection MethodFluorescence

Unit SizeEach

Conteúdo e armazenamento

Store at room temperature and protect from light.

Frequently asked questions (FAQs)

I am using the EZQ Protein Quantitation Kit and the filter paper has curled up during drying. What should I do?

It is not unusual for the filter paper to bend and warp on drying. It is very important that the paper be flat when scanning or the signal will be uneven and give very inaccurate quantitation values. If this is a problem, wet the paper in water and scan it while it is wet.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I am using the EZQ Protein Quantitation Kit and after staining, I notice some large spots or marks on the paper. Why is this?

Protein on the skin (e.g. keratin) will transfer to the filter paper and be stained with the EZQ Protein Quantitation Reagent. We recommend handling the filter paper with tweezers and cleaning the staining dish and tweezers before use to minimize marks.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I spotted my protein samples onto the EZQ protein assay filter paper and I do not have time to complete the assay? Can I stop the procedure and finish later?

Yes, once the samples have dried onto the filter paper, the filter paper can be stored and stained at a later date. After the staining procedure is complete, the signal is very stable, so the paper can be dried again and scanned at a later date as well. The paper can be scanned dry or after dipping it in water. If storing the paper before staining, we recommend storing it in a plastic bag to prevent contamination that could affect the staining pattern.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I ran out of filter paper for the EZQ Protein Quantitation Kit. Can I just cut any filter paper to fit inside the microplate?

No. The filter paper that is supplied with the EZQ Protein Quantitation Kit is a very specific filter paper and the identity is proprietary. Most lab filter papers will not provide similar results as the paper provided in the kit, so substitutions are not recommended.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My buffer or components of my buffer are not listed in the compatibility table for my protein assay. What should I do?

You can test the tolerance of the assay for your specific buffer formulation. For in-house generated compatibility information, substances were considered compatible at the indicated concentration in the Standard Test Tube Protocol (found in the manual for each protein assay) if the error in protein concentration estimation caused by the presence of the substance was less than or equal to 10%. The substances were tested using WR prepared immediately before each experiment. Blank-corrected 562nm absorbance measurements (for a 1000µg/mL BSA standard + substance) were compared to the net 562nm measurements of the same standard prepared in 0.9% saline.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all EZQ™ Protein Quantitation Kit FAQs

Citações e referências (7)

Citações e referências

Abstract

Induced paternal effects mimic cytoplasmic incompatibility in Drosophila.

Authors:Clark ME, Heath BD, Anderson CL, Karr TL

Journal:Genetics

PubMed ID:16489228

'Wolbachia is an intracellular microbe found in a wide diversity of arthropod and filarial nematode hosts. In arthropods these common bacteria are reproductive parasites that manipulate central elements of their host''s reproduction to increase their own maternal transmission in one of several ways. Cytoplasmic incompatibility (CI) is one such manipulation ... More

A novel approach to tag and identify geranylgeranylated proteins.

Authors:Chan LN, Hart C, Guo L, Nyberg T, Davies BS, Fong LG, Young SG, Agnew BJ, Tamanoi F,

Journal:Electrophoresis

PubMed ID:19784953

A recently developed proteomic strategy, the

Gamma-secretase is a functional component of phagosomes.

Authors:Jutras I, Laplante A, Boulais J, Brunet S, Thinakaran G, Desjardins M

Journal:J Biol Chem

PubMed ID:16103123

Gamma-secretase is a high molecular mass protein complex that catalyzes the intramembrane cleavage of its protein substrates. Two proteins involved in phagocytosis, CD44 and the low density lipoprotein receptor-related protein, are gamma-secretase substrates, suggesting that this complex might regulate some aspects of phagocytosis. Our results indicate that the four components ... More

Mechanism of intramembrane proteolysis investigated with purified rhomboid proteases.

Authors:Lemberg MK, Menendez J, Misik A, Garcia M, Koth CM, Freeman M

Journal:EMBO J

PubMed ID:15616571

Intramembrane proteases have the unusual property of cleaving peptide bonds within the lipid bilayer, an environment not obviously suited to a water-requiring hydrolysis reaction. These enzymes include site-2 protease, gamma-secretase/presenilin, signal peptide peptidase and the rhomboids, and they have a wide range of cellular functions. All have multiple transmembrane domains ... More

A rapid solid-phase fluorescence-based protein assay for quantitation of protein electrophoresis samples containing detergents, chaotropes, dyes, and reducing agents.

Authors:Agnew BJ, Murray D, Patton WF

Journal:Electrophoresis

PubMed ID:15300765

A new solid-phase, fluorescence-based protein assay was developed that quantifies proteins in the presence of detergents, urea and reducing agents (one-dimensional sodium dodecyl sulfate (1-D SDS) lysis buffers and urea isoelectric focusing (IEF) buffers). A specially designed 96-well microplate facilitates application of protein samples to the assay paper and allows ... More

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