The thiol-reactive Rhodamine Green™-X, succinimidyl ester can be used to create green-fluorescent bioconjugates with excitation/emission maxima ∼502/527 nm. This dyeLeia mais
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Número do catálogo
Quantity
R6113
conhecido também como R-6113
5 mg
Número do catálogo R6113
conhecido também como R-6113
Preço (BRL)
-
Quantity:
5 mg
The thiol-reactive Rhodamine Green™-X, succinimidyl ester can be used to create green-fluorescent bioconjugates with excitation/emission maxima ∼502/527 nm. This dye is the nonsulfonated analog of the Alexa Fluor™ 488 dye.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Chemical ReactivityAmine
Emission527
Excitation502
Label or DyeRhodamine Green™
Product TypeSuccinimidyl Ester
Quantity5 mg
Reactive MoietyActive Ester, Succinimidyl Ester
Shipping ConditionRoom Temperature
Label TypeClassic Dyes
Product LineRhodamine Green
Unit SizeEach
Conteúdo e armazenamento
Store in freezer (-5 to -30°C) and protect from light.
Frequently asked questions (FAQs)
What is the excitation and emission wavelength for rhodamine?
Rhodamine is a generic term for a wide variety of cationic dyes whose fluorescence emission can range from green, orange to red. The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (EC; cm-1 M-1), for various rhodamine dyes (data derived with dye dissolved in methanol).
Flow cytometric quantitation of human opsonin-dependent phagocytosis and oxidative burst responses to meningococcal antigens.
Authors:Lehmann AK, Halstensen A, Bassøe CF
Journal:Cytometry
PubMed ID:9845434
'A one-step flow cytometric (FCM) assay has been developed to quantify both opsonin- and antigen-dependent phagocytosis and intraphagocyte oxidative burst responses. Meningococcal outer membrane structures (OMV) were adsorbed to fluorescent polystyrene beads, opsonized with serum, and exposed to leukocytes. FCM parameters of phagocytosis were evaluated in combinations with oxidative burst ... More
Ultrasensitive detection and identification of fluorescent molecules by FCS: impact for immunobiology.
Authors:Földes-Papp Z, Demel U, Tilz GP
Journal:Proc Natl Acad Sci U S A
PubMed ID:11572995
'An experimental application of fluorescence correlation spectroscopy is presented for the detection and identification of fluorophores and auto-Abs in solution. The recording time is between 2 and 60 sec. Because the actual number of molecules in the unit volume (confocal detection volume of about 1 fl) is integer or zero, ... More
Nonperturbing fluorescent labeling of polysaccharides.
Authors:Meunier F, Wilkinson KJ
Journal:Biomacromolecules
PubMed ID:12099834
'A fluorescent labeling procedure, which does not perturb macromolecular conformations, was employed to bind a rhodamine derivative to the reducing end of several water-soluble polysaccharides by reductive amination in the presence of sodium cyanoborohydride. Fluorescence correlation spectroscopy, atomic force microscopy, and size exclusion chromatography were used to demonstrate that the ... More
Comparison of sieving matrices for on-the-fly fluorescence lifetime detection of dye-labeled DNA fragments.
Authors:Li L, McGown LB
Journal:Fresenius J Anal Chem
PubMed ID:11293703
'Commercially available, replaceable sieving matrices and their solvent modulated forms were evaluated for use in on-the-fly fluorescence lifetime detection of dye-labeled DNA fragments in capillary electrophoresis. The fragments were labeled with dyes that can be excited by the 488 nm line of an argon ion laser and have lifetimes in ... More
Biological and chemical applications of fluorescence correlation spectroscopy: a review.