Invitrogen™

Streptavidin, Alexa Fluor™ 546 conjugate

Número do catálogo: S11225
Invitrogen™

Streptavidin, Alexa Fluor™ 546 conjugate

Número do catálogo: S11225
Número do catálogo
S11225
Tamanho da unidade
1 mg
Número do catálogoTamanho da unidadePreço (BRL)
S112251 mgEntre em contato ›
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Alexa Fluor™ 546 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor™ 546 Streptavidin Conjugates:
Alexa Fluor™ 546 streptavidin conjugate has Ex/Em maxima of ∼ (556/573)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor™ Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook
For Research Use Only. Not for use in diagnostic procedures.

Especificações

Conjugate
Alexa Fluor 546
Shipping Condition
Room Temperature
Form
Solid
Product Line
Alexa Fluor™
Product Type
Streptavidin Conjugate (fluorescent)
Label or Dye
Alexa Fluor Dyes

Conteúdo e armazenamento

Store in freezer (-5 to -30°C) and protect from light.

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